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Automated maintenance of embryonic stem cell cultures

  1. Stefanie Terstegge1,2,
  2. Iris Laufenberg2,
  3. Jörg Pochert3,
  4. Sabine Schenk1,2,
  5. Joseph Itskovitz-Eldor4,
  6. Elmar Endl5,
  7. Oliver Brüstle1,2,*

Article first published online: 7 SEP 2006

DOI: 10.1002/bit.21061

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 96, Issue 1, pages 195–201, 1 January 2007

Additional Information

How to Cite

Terstegge, S., Laufenberg, I., Pochert, J., Schenk, S., Itskovitz-Eldor, J., Endl, E. and Brüstle, O. (2007), Automated maintenance of embryonic stem cell cultures. Biotechnology and Bioengineering, 96: 195–201. doi: 10.1002/bit.21061

Author Information

  1. 1

    Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn and Hertie Foundation, Bonn, Germany; telephone +49 228 6885 500; fax +49 228 6885 501

  2. 2

    Life & Brain GmbH, Bonn, Germany

  3. 3

    Hamilton Life Science Robotics, Bonaduz, Switzerland

  4. 4

    Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel

  5. 5

    Institute of Molecular Medicine and Experimental Immunology, University of Bonn Medical Center, Bonn, Germany

*Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn and Hertie Foundation, Bonn, Germany; telephone +49 228 6885 500; fax +49 228 6885 501

Publication History

  1. Issue published online: 28 NOV 2006
  2. Article first published online: 7 SEP 2006
  3. Manuscript Accepted: 1 JUN 2006
  4. Manuscript Received: 9 DEC 2005

Funded by

  • ESTOOLS (European Commission 6th Framework Programme). Grant Number: LSHG-CT-2006-018739
  • Hertie Foundation
  • DFG. Grant Number: BR 1337/3-2

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Keywords:

  • embryonic stem cell;
  • cell culture standardization;
  • cell culture automation;
  • cell growth;
  • pluripotency;
  • screening

Abstract

Embryonic stem cell (ESC) technology provides attractive perspectives for generating unlimited numbers of somatic cells for disease modeling and compound screening. A key prerequisite for these industrial applications are standardized and automated systems suitable for stem cell processing. Here we demonstrate that mouse and human ESC propagated by automated culture maintain their mean specific growth rates, their capacity for multi-germlayer differentiation, and the expression of the pluripotency-associated markers SSEA-1/Oct-4 and Tra-1-60/Tra-1-81/Oct-4, respectively. The feasibility of ESC culture automation may greatly facilitate the use of this versatile cell source for a variety of biomedical applications. Biotechnol. Bioeng. 2007;96: 195–201. © 2006 Wiley Periodicals, Inc.

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