Article

A simplified bioprocess for human alpha-fetoprotein production from inclusion bodies

  1. Susanna S.J. Leong1,2,
  2. Anton P.J. Middelberg1,*

Article first published online: 17 NOV 2006

DOI: 10.1002/bit.21271

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 97, Issue 1, pages 99–117, 1 May 2007

Additional Information

How to Cite

Leong, S. S.J. and Middelberg, A. P.J. (2007), A simplified bioprocess for human alpha-fetoprotein production from inclusion bodies. Biotechnol. Bioeng., 97: 99–117. doi: 10.1002/bit.21271

Author Information

  1. 1

    Centre for Biomolecular Engineering, Division of Chemical Engineering, The University of Queensland, St. Lucia, Queensland 4072, Australia; telephone: +61-7- 3346 8784; fax: +61-7-3346-8783

  2. 2

    Department of Chemical Engineering, University of Cambridge, Cambridge, United Kingdom

*Centre for Biomolecular Engineering, Division of Chemical Engineering, The University of Queensland, St. Lucia, Queensland 4072, Australia; telephone: +61-7- 3346 8784; fax: +61-7-3346-8783

Publication History

  1. Issue published online: 27 MAR 2007
  2. Article first published online: 17 NOV 2006
  3. Manuscript Accepted: 6 NOV 2006
  4. Manuscript Received: 3 AUG 2006

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (378K)

Cited in:

CrossRef

This article has been cited by:

  1. 1
    Elena Dudich, Igor Dudich, Lidia Semenkova, Sergey Benevolensky, Elena Morozkina, Aleksey Marchenko, Sergey Zatcepin, Dmitry Dudich, Galina Soboleva, Luidmila Khromikh, Olga Roslovtceva, Eduard Tatulov, Engineering of the Saccharomyces cerevisiae yeast strain with multiple chromosome-integrated genes of human alpha-fetoprotein and its high-yield secretory production, purification, structural and functional characterization, Protein Expression and Purification, 2012, 84, 1, 94

    CrossRef

  2. 2
    E. S. Zubkova, L. N. Semenkova, I. V. Dudich, E. I. Dudich, L. M. Khromykh, P. I. Makarevich, Y. V. Parfyonova, M. Yu. Menshikov, Recombinant human alpha-fetoprotein as a regulator of adipose tissue stromal cell activity, Russian Journal of Bioorganic Chemistry, 2012, 38, 5, 459

    CrossRef

  3. 3
    Xiang Li, Susanna Su Jan Leong, A chromatography-focused bioprocess that eliminates soluble aggregation for bioactive production of a new antimicrobial peptide candidate, Journal of Chromatography A, 2011, 1218, 23, 3654

    CrossRef

  4. 4
    Anindya Basu, Wei Ning Chen, Susanna Su Jan Leong, A rational design for hepatitis B virus X protein refolding and bioprocess development guided by second virial coefficient studies, Applied Microbiology and Biotechnology, 2011, 90, 1, 181

    CrossRef

  5. 5
    Olga A. Sharapova, Maria S. Yurkova, Daniela K. Laurinavichyute, Svetlana M. Andronova, Alexey N. Fedorov, Sergey E. Severin, Evgeny S. Severin, Efficient refolding of a hydrophobic protein with multiple S–S bonds by on-resin immobilized metal affinity chromatography, Journal of Chromatography A, 2011, 1218, 31, 5115

    CrossRef

  6. 6
    O. A. Sharapova, M. S. Yurkova, S. M. Andronova, A. N. Fedorov, S. E. Severin, E. S. Severin, High-efficient renaturation of immobilized recombinant C-terminal fragment of human alpha-fetoprotein, Applied Biochemistry and Microbiology, 2011, 47, 5, 474

    CrossRef

  7. 7
    Anindya Basu, Xiang Li, Susanna Su Jan Leong, Refolding of proteins from inclusion bodies: rational design and recipes, Applied Microbiology and Biotechnology, 2011, 92, 2, 241

    CrossRef

  8. 8
    Sagheer A. Onaizi, Susanna S.J. Leong, Tethering antimicrobial peptides: Current status and potential challenges, Biotechnology Advances, 2011, 29, 1, 67

    CrossRef

  9. 9
    Yu Chen, Susanna Su Jan Leong, High productivity refolding of an inclusion body protein using pulsed-fed size exclusion chromatography, Process Biochemistry, 2010, 45, 9, 1570

    CrossRef

  10. 10
    Olga A. Sharapova, Natalia V. Pozdnykova, Daniela K. Laurinavichyute, Maria S. Yurkova, Galina A. Posypanova, Alexey N. Fedorov, Sergey E. Severin, Evgeny S. Severin, High-efficient expression, refolding and purification of functional recombinant C-terminal fragment of human alpha-fetoprotein, Protein Expression and Purification, 2010, 73, 1, 31

    CrossRef

  11. 11
    O. A. Sharapova, N. V. Pozdnyakova, D. K. Laurinavichyute, M. S. Yurkova, G. A. Posypanova, S. M. Andronova, A. N. Fedorov, S. E. Severin, E. S. Severin, Isolation and characterization of the recombinant human α-fetoprotein fragment corresponding to the C-terminal structural domain, Russian Journal of Bioorganic Chemistry, 2010, 36, 6, 696

    CrossRef

  12. 12
    Erika Ledung, Per-Olov Eriksson, Sven Oscarsson, A strategic crossflow filtration methodology for the initial purification of promegapoietin from inclusion bodies, Journal of Biotechnology, 2009, 141, 1-2, 64

    CrossRef

  13. 13
    Yu Chen, Susanna Su Jan Leong, Adsorptive refolding of a highly disulfide-bonded inclusion body protein using anion-exchange chromatography, Journal of Chromatography A, 2009, 1216, 24, 4877

    CrossRef

  14. 14
    Christina Oberhuber, Yan Ma, Justin Marsh, Neil Rigby, Ursula Smole, Christian Radauer, Stefano Alessandri, Peter Briza, Laurian Zuidmeer, Bernhard Maderegger, Martin Himly, Ana I. Sancho, Ronald van Ree, André Knulst, Christof Ebner, Peter Shewry, E. N. Clare Mills, Klaus Wellner, Heimo Breiteneder, Karin Hoffmann-Sommergruber, Merima Bublin, Purification and characterisation of relevant natural and recombinant apple allergens, Molecular Nutrition & Food Research, 2008, 52, S2
    Direct Link:
Get PDF (378K)