Both FJM and HZ made an equal contribution.
Degradation of supercoiled plasmid DNA within a capillary device†
Article first published online: 17 NOV 2006
Copyright © 2006 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 97, Issue 5, pages 1148–1157, 1 August 2007
How to Cite
Meacle, F.J., Zhang, H., Papantoniou, I., Ward, J.M., Titchener-Hooker, N.J. and Hoare, M. (2007), Degradation of supercoiled plasmid DNA within a capillary device. Biotechnol. Bioeng., 97: 1148–1157. doi: 10.1002/bit.21275
- Issue published online: 22 JUN 2007
- Article first published online: 17 NOV 2006
- Manuscript Accepted: 7 NOV 2006
- Manuscript Received: 23 JUN 2006
- plasmid DNA;
- capillary device;
- shear rate;
- computational fluid dynamics
Supercoiled plasmid DNA is susceptible to fluid stress in large-scale manufacturing processes. A capillary device was used to generate controlled shear conditions and the effects of different stresses on plasmid DNA structure were investigated. Computational fluid dynamics (CFD) analysis was employed to characterize the flow environment in the capillary device and different analytical techniques were used to quantify the DNA breakage. It was found that the degradation of plasmid DNA occurred at the entrance of the capillary and that the shear stress within the capillary did not affect the DNA structure. The degradation rate of plasmids was well correlated with the average elongational strain rate or the pressure drop at the entrance region. The conclusion may also be drawn that laminar shear stress does not play a significant role in plasmid DNA degradation. Biotechnol. Bioeng. 2007; 97: 1148–1157. © 2006 Wiley Periodicals, Inc.