Article

Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose

  1. Alexander V. Gusakov1,*,
  2. Tatyana N. Salanovich1,
  3. Alexey I. Antonov1,
  4. Boris B. Ustinov1,
  5. Oleg N. Okunev2,
  6. Richard Burlingame3,
  7. Mark Emalfarb3,
  8. Marco Baez3,
  9. Arkady P. Sinitsyn1

Article first published online: 12 JAN 2007

DOI: 10.1002/bit.21329

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 97, Issue 5, pages 1028–1038, 1 August 2007

Additional Information

How to Cite

Gusakov, A. V., Salanovich, T. N., Antonov, A. I., Ustinov, B. B., Okunev, O. N., Burlingame, R., Emalfarb, M., Baez, M. and Sinitsyn, A. P. (2007), Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose. Biotechnol. Bioeng., 97: 1028–1038. doi: 10.1002/bit.21329

Author Information

  1. 1

    Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119899, Russia; telephone: (7-495) 939-5966; fax: (7-495) 939-0997

  2. 2

    Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142292, Russia

  3. 3

    Dyadic International, Inc., 140 Intracoastal Pointe Drive, Suite 404, Jupiter, Florida 33477-5094

*Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119899, Russia; telephone: (7-495) 939-5966; fax: (7-495) 939-0997

Publication History

  1. Issue published online: 22 JUN 2007
  2. Article first published online: 12 JAN 2007
  3. Manuscript Accepted: 28 DEC 2006
  4. Manuscript Received: 8 SEP 2006

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Keywords:

  • cellobiohydrolase;
  • cellulase;
  • Chrysosporium lucknowense;
  • endoglucanase;
  • Penicillium sp;
  • Trichoderma reesei

Abstract

An extremely highly active cellobiohydrolase (CBH IIb or Cel6B) was isolated from Chrysosporiumlucknowense UV18-25 culture filtrate. The CBH IIb demonstrated the highest ability for a deep degradation of crystalline cellulose amongst a few cellobiohydrolases tested, including C. lucknowense CBH Ia, Ib, IIa, and Trichodermareesei CBH I and II. Using purified C. lucknowense enzymes (CBH Ia, Ib, and IIb; endoglucanases II and V; β-glucosidase, xylanase II), artificial multienzyme mixtures were reconstituted, displaying an extremely high performance in a conversion of different cellulosic substrates (Avicel, cotton, pretreated Douglas fir wood) to glucose. These mixtures were much or notably more effective in hydrolysis of the cellulosic substrates than the crude multienzyme C. lucknowense preparation and other crude cellulase samples produced by T. reesei and Penicilliumverruculosum. Highly active cellulases are a key factor in bioconversion of plant lignocellulosic biomass to ethanol as an alternative to fossil fuels. Biotechnol. Bioeng. 2007; 97: 1028–1038. © 2007 Wiley Periodicals, Inc.

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