An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture

Authors

  • Philip J. Lee,

    1. Department of Bioengineering, Biomolecular Nanotechnology Center, Berkeley Sensor and Actuator Center, University of California, Berkeley 485 Evans Hall, Berkeley, California 94720-1762; telephone: 510-642-5855; fax: 510-642-5835
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  • Paul J. Hung,

    1. CellASIC Corporation, San Leandro, California
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  • Luke P. Lee

    Corresponding author
    1. Department of Bioengineering, Biomolecular Nanotechnology Center, Berkeley Sensor and Actuator Center, University of California, Berkeley 485 Evans Hall, Berkeley, California 94720-1762; telephone: 510-642-5855; fax: 510-642-5835
    • Department of Bioengineering, Biomolecular Nanotechnology Center, Berkeley Sensor and Actuator Center, University of California, Berkeley 485 Evans Hall, Berkeley, California 94720-1762; telephone: 510-642-5855; fax: 510-642-5835
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Abstract

Primary hepatocytes represent a physiologically relevant model for drug toxicity screening. Here, we created a biologically inspired artificial liver sinusoid with a microfluidic endothelial-like barrier having mass transport properties similar to the liver acinus. This unit consisted of a cord of hepatocytes (50 × 30 × 500 µm) fed by diffusion of nutrients across the microfluidic endothelial-like barrier from a convective transport vessel (10 nL/min). This configuration sustained rat and human hepatocytes for 7 days without an extracellular matrix (ECM) coating. Experiments with the metabolism mediated liver toxicant diclofenac showed no hepatotoxicity after 4 h and an IC50 of 334 ± 41 µM after 24 h. Biotechnol. Bioeng. 2007; 97: 1340–1346. © 2007 Wiley Periodicals, Inc.

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