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Butanol production from agricultural residues: Impact of degradation products on Clostridium beijerinckii growth and butanol fermentation

Authors

  • Thaddeus Ezeji,

    1. Biotechnology & Bioengineering Group, Department of Food Science & Human Nutrition, University of Illinois 1207 W Gregory Drive, Urbana, Illinois 61801
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  • Nasib Qureshi,

    1. United States Department of Agriculture, National Center for Agricultural Utilization Research, Fermentation Biotechnology, 1815 N University Street, Peoria, Illinois 61604
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  • Hans P. Blaschek

    Corresponding author
    1. Biotechnology & Bioengineering Group, Department of Food Science & Human Nutrition, University of Illinois 1207 W Gregory Drive, Urbana, Illinois 61801
    • Biotechnology & Bioengineering Group, Department of Food Science & Human Nutrition, University of Illinois 1207 W Gregory Drive, Urbana, Illinois 61801
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  • Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture.

Abstract

During pretreatment and hydrolysis of fiber-rich agricultural biomass, compounds such as salts, furfural, hydroxymethyl furfural (HMF), acetic, ferulic, glucuronic, ρ-coumaric acids, and phenolic compounds are produced. Clostridium beijerinckii BA101 can utilize the individual sugars present in lignocellulosic [e.g., corn fiber, distillers dry grain solubles (DDGS), etc] hydrolysates such as cellobiose, glucose, mannose, arabinose, and xylose. In these studies we investigated the effect of some of the lignocellulosic hydrolysate inhibitors associated with C. beijerinckii BA101 growth and acetone–butanol–ethanol (ABE) production. When 0.3 g/L ρ-coumaric and ferulic acids were introduced into the fermentation medium, growth and ABE production by C. beijerinckii BA101 decreased significantly. Furfural and HMF are not inhibitory to C. beijerinckii BA101; rather they have stimulatory effect on the growth of the microorganism and ABE production. Biotechnol. Bioeng. 2007;97:1460–1469. © 2007 Wiley Periodicals, Inc.

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