Communication to the Editor

Silica-immobilized enzymes for multi-step synthesis in microfluidic devices

  1. Heather R. Luckarift1,
  2. Bosung S. Ku2,
  3. Jonathan S. Dordick2,*,
  4. Jim C. Spain3,*

Article first published online: 5 APR 2007

DOI: 10.1002/bit.21447

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 98, Issue 3, pages 701–705, 15 October 2007

Additional Information

How to Cite

Luckarift, H. R., Ku, B. S., Dordick, J. S. and Spain, J. C. (2007), Silica-immobilized enzymes for multi-step synthesis in microfluidic devices. Biotechnol. Bioeng., 98: 701–705. doi: 10.1002/bit.21447

Author Information

  1. 1

    Air Force Research Laboratory, 139 Barnes Drive, Tyndall AFB, Florida 32403

  2. 2

    Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180

  3. 3

    School of Civil and Environmental Engineering, 311 Ferst Drive, Georgia Institute of Technology, Atlanta, Georgia 30332-051

*Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180; telephone: 518-276-2899; fax: 518-276-2207.

Publication History

  1. Issue published online: 27 AUG 2007
  2. Article first published online: 5 APR 2007
  3. Manuscript Accepted: 20 MAR 2007
  4. Manuscript Received: 5 JAN 2007

Funded by

  • Air Force Office of Scientific Research
  • NIH. Grant Number: GM66712

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Keywords:

  • microfluidics;
  • immobilized enzyme;
  • aminophenoxazinone;
  • chips;
  • sequential catalysis

Abstract

The combinatorial synthesis of 2-aminophenoxazin-3-one (APO) in a microfluidic device is reported. Individual microfluidic chips containing metallic zinc, silica-immobilized hydroxylaminobenzene mutase and silica-immobilized soybean peroxidase are connected in series to create a chemo-enzymatic system for synthesis. Zinc catalyzes the initial reduction of nitrobenzene to hydroxylaminobenzene which undergoes a biocatalytic conversion to 2-aminophenol, followed by enzymatic polymerization to APO. Silica-immobilization of enzymes allows the rapid stabilization and integration of the biocatalyst within a microfluidic device with minimal preparation. The system proved suitable for synthesis of a complex natural product (APO) from a simple substrate (nitrobenzene) under continuous flow conditions. Biotechnol. Bioeng. 2007;98: 701–705. © 2007 Wiley Periodicals, Inc.

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