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Three-dimensional fibrous PLGA/HAp composite scaffold for BMP-2 delivery

Authors

  • Hemin Nie,

    1. Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore; telephone: +65-6516-5079; fax: +65-6779-1536
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  • Beng Wee Soh,

    1. Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore; telephone: +65-6516-5079; fax: +65-6779-1536
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  • Yin-Chih Fu,

    1. Orthopaedic Research Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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  • Chi-Hwa Wang

    Corresponding author
    1. Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore; telephone: +65-6516-5079; fax: +65-6779-1536
    2. Molecular Engineering of Biological and Chemical Systems, Singapore-MIT Alliance, Singapore 117576, Singapore
    • Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576, Singapore; telephone: +65-6516-5079; fax: +65-6779-1536.
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Abstract

A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a promising fabrication technique, electrospinning. In vitro release of BMP-2 from these scaffolds, and the attachment ability and viability of marrow derived messenchymal stem cells (MSCs) in the presence of the scaffolds were investigated. The PLGA/HAp composite scaffolds developed in this study exhibit good morphology and it was observed that HAp nanoparticles were homogeneously dispersed inside PLGA matrix within the scaffold. The composite scaffolds allowed sustained (2–8 weeks) release of BMP-2 whose release rate was accelerated with increasing HAp content. It was also shown that BMP-2 protein successfully maintained its integrity and natural conformations after undergoing the process of electrospinning. Cell culture experiments showed that the encapsulation of HAp could enhance cell attachment to scaffolds and lower cytotoxicity. Biotechnol. Bioeng. 2008;99: 223–234. © 2007 Wiley Periodicals, Inc.

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