Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture

Authors

  • Sun Ok Hwang,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea; telephone: +82-42-869-2618; fax: +82-42-869-2610
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  • Gyun Min Lee

    Corresponding author
    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea; telephone: +82-42-869-2618; fax: +82-42-869-2610
    • Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea; telephone: +82-42-869-2618; fax: +82-42-869-2610.
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Abstract

During Chinese hamster ovary (CHO) cell culture for foreign protein production, cells are subjected to programmed cell death (PCD). A rapid death at the end of batch culture is accelerated by nutrient starvation. In this study, type II PCD, autophagy, as well as type I PCD, apoptosis, was found to take place in two antibody-producing CHO cell lines, Ab1 and Ab2, toward the end of batch culture when glucose and glutamine were limiting. The evidence of autophagy was observed from the accumulation of a common autophagic marker, a 16 kDa form of LC3-II during batch culture. Moreover, a significant percentage of the total cells (80% of Ab1 cells and 86% of Ab2 cells) showed autophagic vacuoles containing cytoplasmic material by transmission electron microscopy. An increased level of PARP cleavage and chromosomal DNA fragmentation supported that starvation-induced apoptosis also occurred simultaneously with autophagy. Biotechnol. Bioeng. 2008;99: 678–685. © 2007 Wiley Periodicals, Inc.

Ancillary