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Engineering motility as a phenotypic response to LuxI/R-dependent quorum sensing in Escherichia coli

Authors

  • Lucien E. Weiss,

    1. The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846
    2. Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania
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  • Jonathan P. Badalamenti,

    1. The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846
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  • Lane J. Weaver,

    1. The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846
    2. Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania
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  • Anthony R. Tascone,

    1. The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846
    2. Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802
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  • Paul S. Weiss,

    1. The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846
    2. Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania
    3. Department of Physics, The Pennsylvania State University, University Park, Pennsylvania
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  • Tom L. Richard,

    1. The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846
    2. Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, Pennsylvania
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  • Patrick C. Cirino

    Corresponding author
    1. The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846
    2. Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802
    • The Pennsylvania State University iGEM Group, The Pennsylvania State University, University Park, Pennsylvania 16802; telephone: 814-865-5790; fax: 814-865-7846.
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Abstract

The repertoire of functional outputs interfaced with the LuxI/LuxR quorum sensing system in engineered Escherichia coli has been expanded to include motility via inducible expression of motB. Appropriate choice of ribosome binding site controlling MotB translation was crucial to achieving control over motility. Biotechnol. Biotechnol. Bioeng. 2008;100: 1251–1255. © 2008 Wiley Periodicals, Inc.

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