Article

Preparation and anti-tumor efficiency evaluation of doxorubicin-loaded bacterial magnetosomes: Magnetic nanoparticles as drug carriers isolated from Magnetospirillum gryphiswaldense

  1. Jian-Bo Sun1,
  2. Jin-Hong Duan2,
  3. Shun-Ling Dai2,
  4. Jun Ren3,
  5. Lin Guo4,
  6. Wei Jiang1,
  7. Ying Li1,*

Article first published online: 13 JUN 2008

DOI: 10.1002/bit.22011

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 101, Issue 6, pages 1313–1320, 15 December 2008

Additional Information

How to Cite

Sun, J.-B., Duan, J.-H., Dai, S.-L., Ren, J., Guo, L., Jiang, W. and Li, Y. (2008), Preparation and anti-tumor efficiency evaluation of doxorubicin-loaded bacterial magnetosomes: Magnetic nanoparticles as drug carriers isolated from Magnetospirillum gryphiswaldense. Biotechnol. Bioeng., 101: 1313–1320. doi: 10.1002/bit.22011

Author Information

  1. 1

    State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100094, P.R. China; telephone: +86-10-62731241; fax: +86-10-62732715

  2. 2

    Faculty of Basic Medicine, Peking Union Medical College, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, P.R. China

  3. 3

    Center for Cardiovascular Research and Alternative Medicine, University of Wyoming, Laramie, Wyoming

  4. 4

    College of Chemistry and Chemical Engineering, Nanjing University, Nanjing, P.R. China

*State Key Laboratories for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100094, P.R. China; telephone: +86-10-62731241; fax: +86-10-62732715.

Publication History

  1. Issue published online: 3 NOV 2008
  2. Article first published online: 13 JUN 2008
  3. Accepted manuscript online: 13 JUN 2008 12:00AM EST
  4. Manuscript Accepted: 28 MAY 2008
  5. Manuscript Revised: 14 MAY 2008
  6. Manuscript Received: 10 DEC 2007

Funded by

  • Chinese High Technology Research and Development Program. Grant Numbers: 2006AA02Z233, 2007AA021805
  • Chinese National Science Foundation. Grant Number: 30570023

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Keywords:

  • Magnetospirillum gryphiswaldense;
  • bacterial magnetosomes;
  • nano-particle;
  • doxorubicin;
  • antitumor property;
  • drug carrier

Abstract

Bacterial magnetosomes (BMs) are commonly used as vehicles for certain enzymes, nucleic acids and antibodies, although they have never been considered drug carriers. To evaluate the clinical potential of BMs extracted from Magnetospirillum gryphiswaldense in cancer therapy, doxorubicin (DOX) was loaded onto the purified BMs at a ratio of 0.87 ± 0.08 mg/mg using glutaraldehyde. The DOX-coupled BMs (DBMs) and BMs exhibited uniform sizes and morphology evaluated by TEM. The diameters of DBMs and BMs obtained by AFM were 71.02 ± 6.73 and 34.93 ± 8.24 nm, respectively. The DBMs released DOX slowly into serum and maintained at least 80% stability following 48 h of incubation. In vitro cytotoxic tests showed that the DBMs were cytotoxic to HL60 and EMT-6 cells, manifested as inhibition of cell proliferation and suppression in c-myc expression, consistent with DOX. These observations depicted in vitro antitumor property of DBMs similar to DOX. The approach of coupling DOX to magnetosomes may have clinical potential in anti-tumor drug delivery. Biotechnol. Bioeng. 2008;101: 1313–1320. © 2008 Wiley Periodicals, Inc.

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