A novel system for evaluation of drug mixtures for potential efficacy in treating multidrug resistant cancers
Article first published online: 5 DEC 2008
Copyright © 2008 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 103, Issue 1, pages 187–198, 1 May 2009
How to Cite
Tatosian, D. A. and Shuler, M. L. (2009), A novel system for evaluation of drug mixtures for potential efficacy in treating multidrug resistant cancers. Biotechnol. Bioeng., 103: 187–198. doi: 10.1002/bit.22219
- Issue published online: 23 MAR 2009
- Article first published online: 5 DEC 2008
- Accepted manuscript online: 5 DEC 2008 12:00AM EST
- Manuscript Accepted: 1 DEC 2008
- Manuscript Revised: 17 NOV 2008
- Manuscript Received: 3 AUG 2008
- New York State Office of Science, Technology and Academic Research (NYSTAR) Program
- National Science Foundation. Grant Numbers: BES 0342985, ECS-9876771, ECS-9731293
- Cornell Nano-Scale Science & Technology Facility
Additional supporting information may be found in the online version of this article.
|bit_22219_sm_suppFig1.doc||40K||Supplementary Figure 1: Comparison of Calcein Blue and SYTOX Green Labeling to Determine Viability. SYTOX Green (Molecular Probes) was used to label dead cells, and Calcein Blue to label live cells, in control chip experiments to validate the assay method used in this paper. Representative fluorescent images from the C3A cells (a) and MESSA cells (b) of a chip experiment are displayed, with the Calcein Blue fluorescence shown as blue, and SYTOX green fluorescence shown as green. In these overlayed images, cells labeled with the SYTOX green dead stain also have very low labeling of Calcein Blue. Similarily, cells that are labeled with the Calcein Blue live cell stain have no visible labeling of SYTOX green. These experiments were used to establish the threshold range for Calcein Blue to be used in our image analysis. Scalebar shown is 200 µm.|
|bit_22219_sm_suppFig2.doc||496K||Supplementary Figure 2: Preliminary data (small sample size) from µCCA experiments with DOX at different concentrations for 24 hour exposure (top) and variable time exposure at 10 µM DOX (bottom). A substantial difference in viability in the MESSA cells was determined at 10 µM DOX after 24 hour incubation in the device. This concentration DOX was selected to begin the DOX/Modulator mixture experiments.|
|bit_22219_sm_suppTab1.doc||41K||Supplementary Table 1: A Summary of parameters obtained from the literature, and corresponding design and measured parameters used in the µCCA device. In cases where multiple sources were used for human data, we chose intermediate values. *This device design uses an external reservoir (which acts as a debubbler) with a volume of 200 uL for the other tissues chamber instead of the calculated values. Human data taken from literature (Brown et al. 1997; Chan et al. 1978; Gerlowski and Jain 1983; Plowchalk and Teeguarden 2002).|
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