Design and validation of a pulsatile perfusion bioreactor for 3D high cell density cultures

Authors

  • Julie A. Chouinard,

    1. Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, blvd de l'Université, Sherbrooke, QC, Canada J1K 2R1; telephone: 819-821-8000 ext 62826; fax: 819-821-7955
    2. Research Centre on Aging, Institut Universitaire de Gériatrie de Sherbrooke, Sherbrooke, QC, Canada
    3. Department of Radiobiology and Nuclear Medicine, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, QC, Canada
    Search for more papers by this author
  • Serge Gagnon,

    1. Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, blvd de l'Université, Sherbrooke, QC, Canada J1K 2R1; telephone: 819-821-8000 ext 62826; fax: 819-821-7955
    Search for more papers by this author
  • Marc G. Couture,

    1. Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, blvd de l'Université, Sherbrooke, QC, Canada J1K 2R1; telephone: 819-821-8000 ext 62826; fax: 819-821-7955
    Search for more papers by this author
  • Alain Lévesque,

    1. Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, blvd de l'Université, Sherbrooke, QC, Canada J1K 2R1; telephone: 819-821-8000 ext 62826; fax: 819-821-7955
    Search for more papers by this author
  • Patrick Vermette

    Corresponding author
    1. Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, blvd de l'Université, Sherbrooke, QC, Canada J1K 2R1; telephone: 819-821-8000 ext 62826; fax: 819-821-7955
    2. Research Centre on Aging, Institut Universitaire de Gériatrie de Sherbrooke, Sherbrooke, QC, Canada
    • Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, 2500, blvd de l'Université, Sherbrooke, QC, Canada J1K 2R1; telephone: 819-821-8000 ext 62826; fax: 819-821-7955.
    Search for more papers by this author

Errata

This article is corrected by:

  1. Errata: Erratum: Design and validation of a pulsatile perfusion bioreactor for 3D high cell density cultures Volume 109, Issue 3, 851, Article first published online: 27 September 2011

Abstract

This study presents the design and validation of a pulsatile flow perfusion bioreactor able to provide a suitable environment for 3D high cell density cultures for tissue engineering applications. Our bioreactor system is mobile, does not require the use of traditional cell culture incubators and is easy to sterilize. It provides real-time monitoring and stable control of pH, dissolved oxygen concentration, temperature, pressure, pulsation frequency, and flow rate. In this bioreactor system, cells are cultured in a gel within a chamber perfused by a culture medium fed by hollow fibers. Human umbilical vein endothelial cells (HUVEC) suspended in fibrin were found to be living, making connections and proliferating up to five to six times their initial seeding number after a 48-h culture period. Cells were uniformly dispersed within the 14.40 mm × 17.46 mm × 6.35 mm chamber. Cells suspended in 6.35-mm thick gels and cultured in a traditional CO2 incubator were found to be round and dead. In control experiments carried out in a traditional cell culture incubator, the scarcely found living cells were mostly on top of the gels, while cells cultured under perfusion bioreactor conditions were found to be alive and uniformly distributed across the gel. Biotechnol. Bioeng. 2009; 104: 1215–1223. © 2009 Wiley Periodicals, Inc.

Ancillary