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Article first published online: 24 JUL 2009
This article is a US Government work and, as such, is in the public domain in the United States of America. Published in 2009 by John Wiley & Sons, Ltd.
Biotechnology and Bioengineering
Volume 104, Issue 6, pages 1059–1067, 15 December 2009
How to Cite
Yim, P. B., Clarke, M. L., McKinstry, M., De Paoli Lacerda, S. H., Pease, L. F., Dobrovolskaia, M. A., Kang, H., Read, T. D., Sozhamannan, S. and Hwang, J. (2009), Quantitative characterization of quantum dot-labeled lambda phage for Escherichia coli detection . Biotechnol. Bioeng., 104: 1059–1067. doi: 10.1002/bit.22488
Peter B. Yim and Matthew L. Clarke contributed equally to this work.
This article is a U.S. Goverment work and is in the public domain in the U.S.A.
- Issue published online: 22 OCT 2009
- Article first published online: 24 JUL 2009
- Accepted manuscript online: 24 JUL 2009 12:00AM EST
- Manuscript Accepted: 17 JUL 2009
- Manuscript Revised: 14 JUL 2009
- Manuscript Received: 28 MAY 2009
- Defense Threat Reduction Agency
- Department of Defense of the U.S. Government. Grant Number: 8.10084_08
- bacteria detection;
- electron-spray differential mobility assay
We characterize CdSe/ZnS quantum dot (QD) binding to genetically modified bacteriophage as a model for bacterial detection. Interactions among QDs, lambda (λ) phage, and Escherichia coli are examined by several cross-validated methods. Flow and image-based cytometry clarify fluorescent labeling of bacteria, with image-based cytometry additionally reporting the number of decorated phage bound to cells. Transmission electron microscopy, image-based cytometry, and electrospray differential mobility analysis allow quantization of QDs attached to each phage (4–17 QDs) and show that λ phage used in this study exhibits enhanced QD binding to the capsid by nearly a factor of four compared to bacteriophage T7. Additionally, the characterization methodology presented can be applied to the quantitative characterization of other fluorescent nanocrystal-biological conjugates. Biotechnol. Bioeng. 2009;104: 1059–1067. Published 2009 Wiley Periodicals, Inc.