PEGDA hydrogels with patterned elasticity: Novel tools for the study of cell response to substrate rigidity
Article first published online: 8 OCT 2009
Copyright © 2009 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 105, Issue 3, pages 636–644, 15 February 2010
How to Cite
Nemir, S., Hayenga, H. N. and West, J. L. (2010), PEGDA hydrogels with patterned elasticity: Novel tools for the study of cell response to substrate rigidity. Biotechnol. Bioeng., 105: 636–644. doi: 10.1002/bit.22574
- Issue published online: 23 DEC 2009
- Article first published online: 8 OCT 2009
- Accepted manuscript online: 8 OCT 2009 12:00AM EST
- Manuscript Accepted: 28 SEP 2009
- Manuscript Received: 13 SEP 2009
- substrate rigidity;
- substrate stiffness;
The ability of cells to migrate in response to mechanical gradients (durotaxis) and differential cell behavior in adhesion, spreading, and proliferation in response to substrate rigidity are key factors both in tissue engineering, in which materials must be selected to provide the appropriate mechanical signals, and in studies of mechanisms of diseases such as cancer and atherosclerosis, in which changes in tissue stiffness may inform cell behavior. Using poly(ethylene glycol) diacrylate hydrogels with varying polymer chain length and photolithographic patterning techniques, we are able to provide substrates with spatially patterned, tunable mechanical properties in both gradients and distinct patterns. The hydrogels can be patterned to produce anisotropic structures and exhibit patterned strain under mechanical loading. These hydrogels may be used to study cell response to substrate rigidity in both two and three dimensions and can also be used as a scaffold in tissue-engineering applications. Biotechnol. Bioeng. 2010; 105: 636–644. © 2009 Wiley Periodicals, Inc.