Communication to the Editor

Degassing-assisted patterning of cell culture surfaces†

  1. Chunxiong Luo1,2,
  2. Xiaofang Ni1,3,
  3. Li Liu1,
  4. Shin-ichiro M. Nomura1,
  5. Yong Chen1,4,*

Article first published online: 26 OCT 2009

DOI: 10.1002/bit.22586

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 105, Issue 4, pages 854–859, 1 March 2010

Additional Information

How to Cite

Luo, C., Ni, X., Liu, L., Nomura, S.-i. M. and Chen, Y. (2010), Degassing-assisted patterning of cell culture surfaces. Biotechnology and Bioengineering, 105: 854–859. doi: 10.1002/bit.22586

Author Information

  1. 1

    Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, 606-8507 Kyoto, Japan; telephone: 81-75-753-9755; fax: 81-75-753-9759

  2. 2

    Center for the Theoretical Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China

  3. 3

    Chemistry Department, East China Normal University, Shanghai, China

  4. 4

    Ecole Normale Superieure, Paris, France

*Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, 606-8507 Kyoto, Japan; telephone: 81-75-753-9755; fax: 81-75-753-9759.

  1. †

    Chunxiong Luo and Xiaofang Ni contributed equally to this work.

Publication History

  1. Issue published online: 20 JAN 2010
  2. Article first published online: 26 OCT 2009
  3. Accepted manuscript online: 26 OCT 2009 12:00AM EST
  4. Manuscript Accepted: 8 OCT 2009
  5. Manuscript Revised: 6 OCT 2009
  6. Manuscript Received: 31 JUL 2009

Funded by

  • Japanese Government. Grant Numbers: 19684015, 20034016
  • Chinese Natural Science Foundation. Grant Numbers: 10634010, 10704002, 10721403

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Keywords:

  • surface patterning;
  • soft lithography;
  • cell culture

Abstract

We developed an alternative patterning technique which is capable of producing both topographic and biochemical features for cell culture studies. This technique is based on microaspiration induced with a degassed poly (dimethylsiloxane) (PDMS) mold. After degassing in a rough vacuum chamber and placed on a sample surface, liquid solution can be aspired through channels and cavities created in the PDMS mold. Depending on the composition of the solution and the associated drying or incubation processes, a variety of surface patterns can be produced without applying external pressure. For demonstration, we fabricated agarose gel microwells and biomolecule patterns either on a glass plate or in a cell culture Petri dish, both applicable for cell culture studies. Biotechnol. Bioeng. 2010. 105: 854–859. © 2009 Wiley Periodicals, Inc.

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