Article

Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

  1. Bowen Du1,
  2. Lekh N. Sharma2,
  3. Christopher Becker2,
  4. Shou-Feng Chen2,
  5. Richard A. Mowery3,
  6. G. Peter van Walsum3,*,
  7. C. Kevin Chambliss1,2

Article first published online: 15 JUN 2010

DOI: 10.1002/bit.22829

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 107, Issue 3, pages 430–440, 15 October 2010

Additional Information

How to Cite

Du, B., Sharma, L. N., Becker, C., Chen, S.-F., Mowery, R. A., van Walsum, G. P. and Chambliss, C. K. (2010), Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates. Biotechnol. Bioeng., 107: 430–440. doi: 10.1002/bit.22829

Author Information

  1. 1

    Department of Environmental Science, Baylor University, Waco, Texas

  2. 2

    Department of Chemistry and Biochemistry, Baylor University, Waco, Texas

  3. 3

    Department of Chemical and Biological Engineering, Forest Bioproducts Research Institute, University of Maine, 5737 Jenness Hall Rm. 311, Orono, Maine 04469; telephone: 207-581-2226; fax: 207-581-2323

*Department of Chemical and Biological Engineering, Forest Bioproducts Research Institute, University of Maine, 5737 Jenness Hall Rm. 311, Orono, Maine 04469; telephone: 207-581-2226; fax: 207-581-2323.

Publication History

  1. Issue published online: 20 AUG 2010
  2. Article first published online: 15 JUN 2010
  3. Manuscript Accepted: 24 MAY 2010
  4. Manuscript Received: 6 APR 2010

Funded by

  • U.S. Department of Agriculture, Cooperative State Research Education and Extension Service, National Research Initiative. Grant Numbers: 2005-35504-16335, 2008-35504-04612

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Keywords:

  • lignocellulose;
  • biomass hydrolysates;
  • pretreatment;
  • degradation products;
  • inhibitors

Abstract

A variety of potentially inhibitory degradation products are produced during pretreatment of lignocellulosic biomass. Qualitative and quantitative interrogation of pretreatment hydrolysates is paramount to identifying potential correlations between pretreatment chemistries and microbial inhibition in downstream bioconversion processes. In the present study, corn stover, poplar, and pine feedstocks were pretreated under eight different chemical conditions, which are representative of leading pretreatment processes. Pretreatment processes included: 0.7% H2SO4, 0.07% H2SO4, liquid hot water, neutral buffer solution, aqueous ammonia, lime, lime with oxygen pressurization, and wet oxidation. Forty lignocellulosic degradation products resulting from pretreatment were analyzed using high performance liquid chromatography in combination with UV spectroscopy or tandem mass spectrometry detection (HPLC-PDA-MS/MS) and ion chromatography (IC). Of these compounds, several have been reported to be inhibitory, including furfural, hydroxymethyl furfural, ferulic acid, 3,4-dihydroxybenzaldehyde, syringic acid among others. Formation and accumulation of monitored compounds in hydrolysates is demonstrated to be a function of both the feedstock and pretreatment conditions utilized. Biotechnol. Bioeng. 2010;107: 430–440. © 2010 Wiley Periodicals, Inc.

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