Article

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

  1. Chonglong Wang1,
  2. Sang-Hwal Yoon1,
  3. Asad Ali Shah1,
  4. Young-Ryun Chung1,
  5. Jae-Yean Kim1,
  6. Eui-Sung Choi2,
  7. Jay D. Keasling3,
  8. Seon-Won Kim1,*

Article first published online: 15 JUN 2010

DOI: 10.1002/bit.22831

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 107, Issue 3, pages 421–429, 15 October 2010

Additional Information

How to Cite

Wang, C., Yoon, S.-H., Shah, A. A., Chung, Y.-R., Kim, J.-Y., Choi, E.-S., Keasling, J. D. and Kim, S.-W. (2010), Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway. Biotechnol. Bioeng., 107: 421–429. doi: 10.1002/bit.22831

Author Information

  1. 1

    Division of Applied Life Sciences (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea; telephone: 82-55-751-6522; fax: 82-55-759-9363

  2. 2

    Industrial Biotechnology Research Center, KRIBB, Daejeon, Korea

  3. 3

    Department of Chemical Engineering, University of California, Berkeley, California

*Division of Applied Life Sciences (BK21 Program), EB-NCRC and PMBBRC, Gyeongsang National University, Jinju 660-701, Korea; telephone: 82-55-751-6522; fax: 82-55-759-9363.

Publication History

  1. Issue published online: 20 AUG 2010
  2. Article first published online: 15 JUN 2010
  3. Manuscript Accepted: 2 JUN 2010
  4. Manuscript Revised: 11 MAY 2010
  5. Manuscript Received: 15 MAR 2010

Funded by

  • 21C Frontier Microbial Genomics and Applications Center Program, Ministry of Education, Science & Technology
  • Basic Research Program, Ministry of Education, Science & Technology. Grant Number: 2009-0084490

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Keywords:

  • farnesol;
  • mevalonate pathway;
  • FPP synthase;
  • phosphatase;
  • E. coli

Abstract

Farnesol (FOH) production has been carried out in metabolically engineered Escherichia coli. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, E. coli was metabolically engineered to overexpress ispA and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two-phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant E. coli harboring the pTispA and pSNA plasmids for ispA overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from E. coli without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by E. coli while no detectable FOH production was observed in the endogenous MEP pathway-only control. Biotechnol. Bioeng. 2010;107: 421–429. © 2010 Wiley Periodicals, Inc.

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