Continuous scalable blood filtration device using inertial microfluidics
Article first published online: 29 JUN 2010
Copyright © 2010 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 107, Issue 2, pages 302–311, 1 October 2010
How to Cite
Mach, A. J. and Di Carlo, D. (2010), Continuous scalable blood filtration device using inertial microfluidics. Biotechnol. Bioeng., 107: 302–311. doi: 10.1002/bit.22833
- Issue published online: 2 AUG 2010
- Article first published online: 29 JUN 2010
- Manuscript Accepted: 2 JUN 2010
- Manuscript Revised: 6 MAY 2010
- Manuscript Received: 11 FEB 2010
- blood filtration;
- label-free cell separation;
- size-based cell separation
Cell separation is broadly useful for applications in clinical diagnostics, biological research, and potentially regenerative medicine. Recent attention has been paid to label-free size-based techniques that may avoid the costs or clogging issues associated with centrifugation and mechanical filtration. We present for the first time a massively parallel microfluidic device that passively separates pathogenic bacteria cells from diluted blood with macroscale performance. The device was designed to process large sample volumes in a high-throughput, continuous manner using 40 single microchannels placed in a radial array with one inlet and two rings of outlets. Each single channel consists of a short focusing, gradual expansion and collection region and uses unique differential transit times due to size-dependent inertial lift forces as a method of cell separation. The gradual channel expansion region is shown to manipulate cell equilibrium positions close to the microchannel walls, critical for higher efficiency collection. We demonstrate >80% removal of pathogenic bacteria from blood after two passes of the single channel system. The massively parallel device can process 240 mL/h with a throughput of 400 million cells/min. We expect that this parallelizable, robust, and label-free approach would be useful for filtration of blood as well as for other cell separation and concentration applications from large volume samples. Biotechnol. Bioeng. 2010;107: 302–311. © 2010 Wiley Periodicals, Inc.