Synthesis and utilization of E. coli-encapsulated PEG-based microdroplet using a microfluidic chip for biological application

Authors

  • Kyoung G. Lee,

    1. NEMS-Bio Team, National NanoFab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: 82-42-879-9722; fax: 82-42-879-9609
    2. Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, Yuseong-gu, Daejeon, Republic of Korea
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  • Tae Jung Park,

    1. Bioprocess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Yuseong-gu, Daejeon, Republic of Korea
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  • Song Young Soo,

    1. NEMS-Bio Team, National NanoFab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: 82-42-879-9722; fax: 82-42-879-9609
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  • Kye Won Wang,

    1. Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, Yuseong-gu, Daejeon, Republic of Korea
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  • Byeong I.I. Kim,

    1. NEMS-Bio Team, National NanoFab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: 82-42-879-9722; fax: 82-42-879-9609
    2. Department of Chemical Engineering, Chungnam National University, Yuseong-gu, Daejeon, Republic of Korea
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  • Jae Hong Park,

    1. NEMS-Bio Team, National NanoFab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: 82-42-879-9722; fax: 82-42-879-9609
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  • Chang-Soo Lee,

    1. Department of Chemical Engineering, Chungnam National University, Yuseong-gu, Daejeon, Republic of Korea
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  • Do Hyun Kim,

    1. Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, Yuseong-gu, Daejeon, Republic of Korea
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  • Seok Jae Lee

    Corresponding author
    1. NEMS-Bio Team, National NanoFab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: 82-42-879-9722; fax: 82-42-879-9609
    • NEMS-Bio Team, National NanoFab Center, 335 Gwahangno, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: 82-42-879-9722; fax: 82-42-879-9609.
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Abstract

We report herein an effective strategy for encapsulating Escherichia coli in polyethylene glycol diacrylate (PEGDA) microdroplets using a microfluidic device and chemical polymerization. PEGDA was employed as a reactant due to the biocompatibility, high porosity, and hydrophilic property. The uniform size and shape of microdroplets are obtained in a single-step process using microfluidic device. The size of microdroplets can be controlled through the changing continuous flow rate. The combination of microdroplet generation and chemical polymerization techniques provide unique environment to produce non-toxic ways of fabricating microorganism-encapsulated hydrogel microbeads. Due to these unique properties of micro-sized hydrogel microbeads, the encapsulated E. coli can maintain viability inside of microbeads and green fluorescent protein (GFP) and red fluorescent protein (RFP) genes are efficiently expressed inside of microbeads after isopropyl-β-D-thiogalactopyranoside induction, suggesting that there is no low-molecular weight substrate transfer limitation inside of microbeads. Furthermore, non-toxic, gentle, and outstanding biocompatibility of microbeads, the encapsulated E. coli can be used in various applications including biotransformation, biosensing, bioremediation, and engineering of artificial cells. Biotechnol. Bioeng. 2010;107:747–751. © 2010 Wiley Periodicals, Inc.

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