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Enhanced butyric acid tolerance and bioproduction by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor

Authors

  • Ling Jiang,

    1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, PR China; telephone: +86-20-39380626; fax: +86-20-39380626
    2. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, PR China
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  • Jufang Wang,

    Corresponding author
    1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, PR China; telephone: +86-20-39380626; fax: +86-20-39380626
    • School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, PR China; telephone: +86-20-39380626; fax: +86-20-39380626.
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  • Shizhong Liang,

    1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510640, PR China; telephone: +86-20-39380626; fax: +86-20-39380626
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  • Jin Cai,

    1. Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, PR China; telephone: +86-571-87951220; fax: +86-571-87951220
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  • Zhinan Xu,

    1. Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, PR China; telephone: +86-571-87951220; fax: +86-571-87951220
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  • Peilin Cen,

    1. Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, PR China; telephone: +86-571-87951220; fax: +86-571-87951220
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  • Shangtian Yang,

    1. Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio
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  • Shuang Li

    1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, PR China
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Abstract

Repeated fed-batch fermentation of glucose by Clostridium tyrobutyricum immobilized in a fibrous bed bioreactor (FBB) was successfully employed to produce butyric acid at a high final concentration as well as to adapt a butyric-acid-tolerant strain. At the end of the eighth fed-batch fermentation, the butyric acid concentration reached 86.9 ± 2.17 g/L, which to our knowledge is the highest butyric acid concentration ever produced in the traditional fermentation process. To understand the mechanism and factors contributing to the improved butyric acid production and enhanced acid tolerance, adapted strains were harvested from the FBB and characterized for their physiological properties, including specific growth rate, acid-forming enzymes, intracellular pH, membrane-bound ATPase and cell morphology. Compared with the original culture used to seed the bioreactor, the adapted culture showed significantly reduced inhibition effects of butyric acid on specific growth rate, cellular activities of butyric-acid-forming enzyme phosphotransbutyrylase (PTB) and ATPase, together with elevated intracellular pH, and elongated rod morphology. Biotechnol. Bioeng. 2011; 108:31–40. © 2010 Wiley Periodicals, Inc.

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