Engineering butanol-tolerance in escherichia coli with artificial transcription factor libraries

Authors

  • Ju Young Lee,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea, telephone: 82-42-350-2619; fax: 82-42-350-2610
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  • Kyung Seok Yang,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea, telephone: 82-42-350-2619; fax: 82-42-350-2610
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  • Su A Jang,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea, telephone: 82-42-350-2619; fax: 82-42-350-2610
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  • Bong Hyun Sung,

    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea, telephone: 82-42-350-2619; fax: 82-42-350-2610
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  • Sun Chang Kim

    Corresponding author
    1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea, telephone: 82-42-350-2619; fax: 82-42-350-2610
    • Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea, telephone: 82-42-350-2619; fax: 82-42-350-2610.
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  • Additional supporting information may be found in the online version of this article.

Abstract

Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli. In particular, E. coli growth is severely inhibited by butanol, being almost completely stopped by 1% (vol/vol) butanol. Here we developed a new method to increase the butanol-tolerance of E. coli with artificial transcription factor (ATF) libraries which consist of zinc finger (ZF) DNA-binding proteins and an E. coli cyclic AMP receptor protein (CRP). Using these ATFs, we selected a butanol-tolerant E. coli which can tolerate up to 1.5% (vol/vol) butanol, with a concomitant increase in heat resistance. We also identified genes of E. coli that are associated with the butanol-tolerance. These results show that E. coli can be engineered as a promising host for high-yield butanol production. Biotechnol. Bioeng. 2011; 108:742–749. © 2010 Wiley Periodicals, Inc.

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