Micropatterns of cell adhesive proteins with poly(ethylene oxide)-block-Poly(4-vinylpyridine) diblock copolymer

Authors

  • J. Racine,

    1. Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 3, Research Link, Singapore 117602, Singapore; telephone: 65-68748137; fax: 65-68720785
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  • H. Cheradame,

    1. Equipe Matériaux Polymères aux Interfaces, Université d'Evry—Val-d'Essonne, ICMPE, UMR CNRS 7182, Evry, France
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  • Y.S. Chu,

    1. Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
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  • J.P. Thiery,

    1. Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
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  • I. Rodriguez

    Corresponding author
    1. Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 3, Research Link, Singapore 117602, Singapore; telephone: 65-68748137; fax: 65-68720785
    • Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 3, Research Link, Singapore 117602, Singapore; telephone: 65-68748137; fax: 65-68720785.
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Abstract

Control of cell shape and behavior through the micropattern technique by spatial immobilization of adhesive proteins on a surface has provided novel insights in several aspects of cell biology, such as tissue morphogenesis, cell growth and cell differentiation, and apoptosis. In this work, we present the use of poly(ethylene oxide-block-poly(4-vinylpyridine) (PEO-b-P4VP) as a non-adhesive background to construct micropatterns of cell adhesive proteins. In the method presented, PEO-b-P4VP is used for its antifouling properties and at the same time, as a photosensitive material to define the micropatterns. The irradiation of PEO-b-P4VP with a short wavelength UV light through photolithographic mask, causes the polymer to crosslink and immobilize in the areas exposed. In the areas non-exposed the polymer can be removed. These areas can be subsequent back filled with the adhesive protein of interest to produce the final micropatterned cell chips. Biotechnol. Bioeng. 2011; 108:983–987. © 2010 Wiley Periodicals, Inc.

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