Bioproduction of butanol in bioreactors: New insights from simultaneous in situ butanol recovery to eliminate product toxicity

Authors

  • Adriano Pinto Mariano,

    1. Department of Animal Sciences, Ohio State Agricultural Research and Development Center (OARDC), The Ohio State University, 1680 Madison Avenue, Wooster, Ohio 44691; telephone: +1-330-263-3796; fax: +1-330-263-3949
    2. Laboratory of Optimization, Design and Advanced Control (LOPCA), School of Chemical Engineering—University of Campinas (UNICAMP), Campinas, SP, Brazil
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  • Nasib Qureshi,

    1. United States Department of Agriculture, ARS, National Center for Agricultural Utilization Research, Bioenergy Research Unit, Peoria, Illinois 61604
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  • Rubens Maciel Filho,

    1. Laboratory of Optimization, Design and Advanced Control (LOPCA), School of Chemical Engineering—University of Campinas (UNICAMP), Campinas, SP, Brazil
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  • Thaddeus Chukwuemeka Ezeji

    Corresponding author
    1. Department of Animal Sciences, Ohio State Agricultural Research and Development Center (OARDC), The Ohio State University, 1680 Madison Avenue, Wooster, Ohio 44691; telephone: +1-330-263-3796; fax: +1-330-263-3949
    • Department of Animal Sciences, Ohio State Agricultural Research and Development Center (OARDC), The Ohio State University, 1680 Madison Avenue, Wooster, Ohio 44691; telephone: +1-330-263-3796; fax: +1-330-263-3949.
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  • Mention of trade names or commercial products in this article is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

Abstract

Simultaneous acetone butanol ethanol (ABE) fermentation by Clostridium beijerinckii P260 and in situ product recovery was investigated using a vacuum process operated in two modes: continuous and intermittent. Integrated batch fermentations and ABE recovery were conducted at 37°C using a 14-L bioreactor (7.0 L fermentation volume) containing initial substrate (glucose) concentration of 60 g/L. The bioreactor was connected in series with a condensation system and vacuum pump. Vacuum was applied continuously or intermittently with 1.5 h vacuum sessions separated by 4, 6, and 8 h intervals. A control ABE fermentation experiment was characterized by incomplete glucose utilization due to butanol toxicity to C. beijerinckii P260, while fermentation coupled with in situ recovery by both continuous and intermittent vacuum modes resulted in complete utilization of glucose, greater productivity, improved cell growth, and concentrated recovered ABE stream. These results demonstrate that vacuum technology can be applied to integrated ABE fermentation and recovery even though the boiling point of butanol is greater than that of water. Biotechnol. Bioeng. 2011; 108:1757–1765. © 2011 Wiley Periodicals, Inc.

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