Acceleration of neuronal precursors differentiation induced by substrate nanotopography

Authors

  • Elisa Migliorini,

    1. CNR-IOM Laboratorio TASC, Area Science Park, Basovizza, 34149 Trieste, Italy
    2. CBM S.c.r.l., Area Science Park, Basovizza, 34149 Trieste, Italy
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  • Gianluca Grenci,

    1. CNR-IOM Laboratorio TASC, Area Science Park, Basovizza, 34149 Trieste, Italy
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  • Jelena Ban,

    1. SISSA-International School for Advanced Studies, Via Bonomea 265, Trieste, Italy, 34136; telephone: +39-0403787769; fax: +39-040-3787702
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  • Alessandro Pozzato,

    1. CNR-IOM Laboratorio TASC, Area Science Park, Basovizza, 34149 Trieste, Italy
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  • Massimo Tormen,

    1. CNR-IOM Laboratorio TASC, Area Science Park, Basovizza, 34149 Trieste, Italy
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  • Marco Lazzarino,

    1. CNR-IOM Laboratorio TASC, Area Science Park, Basovizza, 34149 Trieste, Italy
    2. CBM S.c.r.l., Area Science Park, Basovizza, 34149 Trieste, Italy
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  • Vincent Torre,

    1. SISSA-International School for Advanced Studies, Via Bonomea 265, Trieste, Italy, 34136; telephone: +39-0403787769; fax: +39-040-3787702
    2. Italian Institute of Technology, SISSA Unit, Via Morego 30, 16163 Genova, Italy
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  • Maria Elisabetta Ruaro

    Corresponding author
    1. SISSA-International School for Advanced Studies, Via Bonomea 265, Trieste, Italy, 34136; telephone: +39-0403787769; fax: +39-040-3787702
    2. Glance Vision Technologies, Area Science Park, Basovizza, 34149 Trieste, Italy
    • SISSA-International School for Advanced Studies, Via Bonomea 265, Trieste, Italy, 34136; telephone: +39-0403787769; fax: +39-040-3787702.
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Abstract

Embryonic stem (ES) cell differentiation in specific cell lineages is a major issue in cell biology particularly in regenerative medicine. Differentiation is usually achieved by using biochemical factors and it is not clear whether mechanical properties of the substrate over which cells are grown can affect proliferation and differentiation. Therefore, we produced patterns in polydimethylsiloxane (PDMS) consisting of groove and pillar arrays of sub-micrometric lateral resolution as substrates for cell cultures. We analyzed the effect of different nanostructures on differentiation of ES-derived neuronal precursors into neuronal lineage without adding biochemical factors. Neuronal precursors adhered on PDMS more effectively than on glass coverslips. We demonstrated that neuronal yield was enhanced by increasing pillars height from 35 to 400 nm. On higher pillar neuronal differentiation reaches ∼80% 96 h after plating and the largest differentiation enhancement of pillars over flat PDMS was observed during the first 6 h of culture. We conclude that PDMS nanopillars accelerate and increase neuronal differentiation. Biotechnol. Bioeng. 2011;108: 2736–2746. © 2011 Wiley Periodicals, Inc.

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