Recycling and adaptation of Klebsormidium flaccidum microalgae for the sustained production of gold nanoparticles

Authors

  • Si Amar Dahoumane,

    1. Univ Paris Diderot, Sorbonne Paris Cité, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), UMR 7086, CNRS, 15 rue Jean de Baïf, F-75205 Paris Cedex 13, France; telephone: 33-11-57-27-87-64; fax: 33-11-57-27-72-63
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  • Chakib Djediat,

    1. Muséum National d'Histoire Naturelle, Département RDDM, FRE 3206, USM 505, 57 rue Cuvier, F-75005 Paris, France
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  • Claude Yéprémian,

    1. Muséum National d'Histoire Naturelle, Département RDDM, FRE 3206, USM 505, 57 rue Cuvier, F-75005 Paris, France
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  • Alain Couté,

    1. Muséum National d'Histoire Naturelle, Département RDDM, FRE 3206, USM 505, 57 rue Cuvier, F-75005 Paris, France
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  • Fernand Fiévet,

    1. Univ Paris Diderot, Sorbonne Paris Cité, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), UMR 7086, CNRS, 15 rue Jean de Baïf, F-75205 Paris Cedex 13, France; telephone: 33-11-57-27-87-64; fax: 33-11-57-27-72-63
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  • Thibaud Coradin,

    1. UPMC Univ Paris 06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 place Marcellin Berthelot, F-75005 Paris, France
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  • Roberta Brayner

    Corresponding author
    1. Univ Paris Diderot, Sorbonne Paris Cité, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), UMR 7086, CNRS, 15 rue Jean de Baïf, F-75205 Paris Cedex 13, France; telephone: 33-11-57-27-87-64; fax: 33-11-57-27-72-63
    • Univ Paris Diderot, Sorbonne Paris Cité, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), UMR 7086, CNRS, 15 rue Jean de Baïf, F-75205 Paris Cedex 13, France; telephone: 33-11-57-27-87-64; fax: 33-11-57-27-72-63.
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Abstract

Targeting the development of cell-based bioreactors for the production of metal nanoparticles, the possibility to perform the sustained synthesis of colloidal gold using Klebsormidium flaccidum green algae was studied. A first strategy relying on successive growth/reduction/reseeding recycling steps demonstrated maintained biosynthesis capability of the microalgae but limitation in metal content due to toxic effects. An alternative approach consisting of progressive gold salt addition revealed to be suitable to favor cell adaptation to larger metal concentrations and supported particle release over month periods. Biotechnol. Bioeng. 2012;109: 284–288. © 2011 Wiley Periodicals, Inc.

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