Application of a reaction model to improve calculation of the sugar recovery standard for sugar analysis

Authors

  • Jiacheng Shen,

    Corresponding author
    1. Center for Environmental Research and Technology, Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, California 92507; telephone: +208-423-6565; fax: +208-423-6555
    2. Northwest Irrigation and Soils Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Kimberly, Idaho
    • Center for Environmental Research and Technology, Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, California 92507; telephone: +208-423-6565; fax: +208-423-6555.
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  • Charles E. Wyman

    1. Center for Environmental Research and Technology, Bourns College of Engineering, University of California, 1084 Columbia Avenue, Riverside, California 92507; telephone: +208-423-6565; fax: +208-423-6555
    2. Chemical and Environmental Engineering Department, Bourns College of Engineering, University of California, Riverside, California
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Abstract

A kinetic model was applied to improve determination of the sugar recovery standard (SRS) for biomass analysis. Three sets of xylose (0.10–1.00 g/L and 0.999–19.995 g/L) and glucose (0.206–1.602 g/L) concentrations were measured by HPLC following reaction of each for 1 h. Then, parameters in a kinetic model were fit to the resulting sugar concentration data, and the model was applied to predict the initial sugar concentrations and the best SRS value (SRSp). The initial sugar concentrations predicted by the model agreed with the actual initial sugar concentrations. Although the SRSe calculated directly from experimental data oscillated considerably with sugar concentration, the SRSp trend was smooth. Statistical analysis of errors and application of the F-test confirmed that application of the model reduced experimental errors in SRSe. Reference SRSe values are reported for the three series of concentrations. Biotechnol. Bioeng. 2012;109: 300–305. © 2011 Wiley Periodicals, Inc.

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