Article

Cell transfer printing from patterned poly(ethylene glycol)-oleyl surfaces to biological hydrogels for rapid and efficient cell micropatterning

  1. Toshiaki Takano1,
  2. Satoshi Yamaguchi1,
  3. Erika Matsunuma1,
  4. Senori Komiya2,
  5. Masashige Shinkai1,
  6. Toshiaki Takezawa3,
  7. Teruyuki Nagamune1,2,*

Article first published online: 24 AUG 2011

DOI: 10.1002/bit.23284

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 109, Issue 1, pages 244–251, January 2012

Additional Information

How to Cite

Takano, T., Yamaguchi, S., Matsunuma, E., Komiya, S., Shinkai, M., Takezawa, T. and Nagamune, T. (2012), Cell transfer printing from patterned poly(ethylene glycol)-oleyl surfaces to biological hydrogels for rapid and efficient cell micropatterning. Biotechnol. Bioeng., 109: 244–251. doi: 10.1002/bit.23284

Author Information

  1. 1

    Department of Chemistry & Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; telephone: +81-3-5841-7328; fax: +81-3-5841-8657

  2. 2

    Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

  3. 3

    Laboratory of Animal Cell Biology, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan

*Department of Chemistry & Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan; telephone: +81-3-5841-7328; fax: +81-3-5841-8657.

Publication History

  1. Issue published online: 17 NOV 2011
  2. Article first published online: 24 AUG 2011
  3. Accepted manuscript online: 1 AUG 2011 07:14AM EST
  4. Manuscript Accepted: 22 JUL 2011
  5. Manuscript Revised: 2 JUL 2011
  6. Manuscript Received: 7 MAY 2011

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Keywords:

  • cell stamping;
  • PEG lipid;
  • extracellular matrix;
  • cell microarray;
  • endothelial cell

Abstract

Cell transfer printing from patterned poly(ethylene glycol)-oleyl surfaces onto biological hydrogel sheets is investigated herein, as a new cell stamping method for both cell microarray and tissue engineering. By overlaying a hydrogel sheet on the cells immobilized on the poly(ethylene glycol)-oleyl surface and successively peeling it off, the immobilized cells were transferred onto a hydrogel sheet because the adhesive interaction between the cells and the hydrogel was stronger than that between the cells and the poly(ethylene glycol)-oleyl surface. Four types of human cell could be efficiently transferred onto a rigid collagen sheet. The transfer printing ratios, for all cells, were above 80% and achieved within 90 min. A cell microarray was successfully prepared on a collagen gel sheet using the present stamping method. We have also demonstrated that the transferred pattern of endothelial cells is transformed to the patterned tube-like structure on the reconstituted basement membrane matrix. Finally, the patterns of two types of endothelial cell are shown to be easily prepared on the matrix, and the desired tube-like structures, including the orderly pattern of the two different cells, were formed spontaneously. Thus, the present poly(ethylene glycol)-oleyl coated substrates are useful for rapid and efficient cell stamping, in the preparation of multi-cellular pattern on extracellular matrices. Biotechnol. Bioeng. 2012;109: 244–251. © 2011 Wiley Periodicals, Inc.

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