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Organoclay-assisted interfacial polymerization for microfluidic production of monodisperse PEG-microdroplets and in situ encapsulation of E. coli

Authors

  • Kye Won Wang,

    1. Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea
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  • Kyoung G. Lee,

    1. Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea
    2. Center for Nanobio Integration and Convergence Engineering, National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: +82-42-879-9683; fax: +82-42-879-9609
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  • Tae Jung Park,

    1. Bioprocess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea
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  • Young-Chul Lee,

    1. Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea
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  • Ji-Won Yang,

    1. Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea
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  • Do Hyun Kim,

    1. Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea
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  • Seok Jae Lee,

    Corresponding author
    1. Center for Nanobio Integration and Convergence Engineering, National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: +82-42-879-9683; fax: +82-42-879-9609
    • Center for Nanobio Integration and Convergence Engineering, National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea; telephone: +82-42-879-9683; fax: +82-42-879-9609.
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  • Jung Youn Park

    1. Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), 408-1 Sirang-ri, Gijang, Busan 619-705, Republic of Korea
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Abstract

We developed a novel one-pot synthetic strategy for preparing monodisperse polyethylene glycol diacrylate (PEGDA) microdroplets via organoclay-assisted interfacial polymerization approach for Escherichia coli encapsulation. Based on the mechanism of spontaneous and rapid polymerization of PEGDA precursor solution with Mg-organoclay, the prepared PEGDA microdroplets have uniform size and fine round shape, with size range of 74–118 µm. The size of microdroplets can be controlled through the changing continuous phase flow rate. Organoclay-assisted polymerization method provides a unique environment to produce non-toxic ways of fabricating microorganism encapsulated microdroplets and to prohibit microdroplets merge during the processes. Furthermore, we successfully carried out to entrap E. coli inside of the PEGDA microdroplets. E. coli expressing a green fluorescent protein shows a good viability inside the PEGDA microdroplets. The in situ microfluidic synthetic method provides a novel approach for the preparation of monodisperse PEGDA microdroplets via a one-pot route. Biotechnol. Bioeng. 2012;109: 289–294. © 2011 Wiley Periodicals, Inc.

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