Construction of a recombinant Trichoderma reesei strain expressing Aspergillus aculeatus β-glucosidase 1 for efficient biomass conversion

Authors

  • Hikaru Nakazawa,

    1. Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka 940-2188, Japan; telephone: +81-258-47-9407; fax: +81-258-47-9400
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  • Tetsushi Kawai,

    1. Tsukuba Laboratory, Japan Bioindustry Association, AIST Tsukuba Central, Higashi, Tsukuba, Ibaraki, Japan
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  • Noriko Ida,

    1. Tsukuba Laboratory, Japan Bioindustry Association, AIST Tsukuba Central, Higashi, Tsukuba, Ibaraki, Japan
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  • Yosuke Shida,

    1. Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka 940-2188, Japan; telephone: +81-258-47-9407; fax: +81-258-47-9400
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  • Yoshinori Kobayashi,

    1. Tsukuba Laboratory, Japan Bioindustry Association, AIST Tsukuba Central, Higashi, Tsukuba, Ibaraki, Japan
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  • Hirofumi Okada,

    1. Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka 940-2188, Japan; telephone: +81-258-47-9407; fax: +81-258-47-9400
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  • Shuji Tani,

    1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Gakuen-cho, Naka-ku, Sakai, Osaka, Japan
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  • Jun-ichi Sumitani,

    1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Gakuen-cho, Naka-ku, Sakai, Osaka, Japan
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  • Takashi Kawaguchi,

    1. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Gakuen-cho, Naka-ku, Sakai, Osaka, Japan
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  • Yasushi Morikawa,

    1. Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka 940-2188, Japan; telephone: +81-258-47-9407; fax: +81-258-47-9400
    2. Tsukuba Laboratory, Japan Bioindustry Association, AIST Tsukuba Central, Higashi, Tsukuba, Ibaraki, Japan
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  • Wataru Ogasawara

    Corresponding author
    1. Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka 940-2188, Japan; telephone: +81-258-47-9407; fax: +81-258-47-9400
    • Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka 940-2188, Japan; telephone: +81-258-47-9407; fax: +81-258-47-9400.
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Abstract

To develop a Trichoderma reesei strain appropriate for the saccharification of pretreated cellulosic biomass, a recombinant T. reesei strain, X3AB1, was constructed that expressed an Aspergillus aculeatus β-glucosidase 1 with high specific activity under the control of the xyn3 promoter. The culture supernatant from T. reesei X3AB1 grown on 1% Avicel as a carbon source had 63- and 25-fold higher β-glucosidase activity against cellobiose compared to that of the parent strain PC-3-7 and that of the T. reesei recombinant strain expressing an endogenous β-glucosidase I, respectively. Further, the xylanase activity was 30% lower than that of PC-3-7 due to the absence of xyn3. X3AB1 grown on 1% Avicel-0.5% xylan medium produced 2.3- and 3.3-fold more xylanase and β-xylosidase, respectively, than X3AB1 grown on 1% Avicel. The supernatant from X3AB1 grown on Avicel and xylan saccharified NaOH-pretreated rice straw efficiently at a low enzyme dose, indicating that the strain has good potential for use in cellulosic biomass conversion processes. Biotechnol. Bioeng. 2012;109: 92–99. © 2011 Wiley Periodicals, Inc.

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