Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications

Authors

  • Mike Fischback,

    1. Chemical Engineering Department, Washington State University, Pullman, Washington 99164-2710; telephone: 1-509-335-3786; fax: 1-509-335-4806
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  • Ki Young Kwon,

    1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon, Republic of Korea
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  • Inseon Lee,

    1. Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea; telephone: 82-2-3290-4850; fax: 82-2-926-6102
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  • Su Jeong Shin,

    1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon, Republic of Korea
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  • Hyun Gyu Park,

    1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon, Republic of Korea
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  • Byoung Chan Kim,

    1. Environment Sensor System Research Center, Korea Institute of Science and Technology, Seoul, Republic of Korea
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  • Yongchai Kwon,

    1. Graduate School of Energy and Environment, Seoul National University of Science and Technology, Seoul, Korea
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  • Hee-Tae Jung,

    1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon, Republic of Korea
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  • Jungbae Kim,

    Corresponding author
    1. Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea; telephone: 82-2-3290-4850; fax: 82-2-926-6102
    • Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea; telephone: 82-2-3290-4850; fax: 82-2-926-6102.
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  • Su Ha

    Corresponding author
    1. Chemical Engineering Department, Washington State University, Pullman, Washington 99164-2710; telephone: 1-509-335-3786; fax: 1-509-335-4806
    • Chemical Engineering Department, Washington State University, Pullman, Washington 99164-2710; telephone: 1-509-335-3786; fax: 1-509-335-4806.
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Abstract

Enzymatic biofuel cells (BFC) have a great potential as a small power source, but their practical applications are being hampered by short lifetime and low power density. This study describes the direct immobilization of glucose oxidase (GOx) onto the carbon paper in the form of highly stable and active enzyme precipitation coatings (EPCs), which can improve the lifetime and power density of BFCs. EPCs were fabricated directly onto the carbon paper via a three-step process: covalent attachment (CA), enzyme precipitation, and chemical crosslinking. GOx-immobilized carbon papers via the CA and EPC approaches were used as an enzyme anode and their electrochemical activities were tested under the BFC-operating mode. The BFCs with CA and EPC enzyme anodes produced the maximum power densities of 50 and 250 µW/cm2, respectively. The BFC with the EPC enzyme anode showed a stable current density output of >700 µA/cm2 at 0.18 V under continuous operation for over 45 h. When a maple syrup was used as a fuel under ambient conditions, it also produced a stable current density of >10 µA/cm2 at 0.18 V for over 25 h. It is anticipated that the direct immobilization of EPC on hierarchical-structured electrodes with a large surface area would further improve the power density of BFCs that can make their applications more feasible. Biotechnol. Bioeng. 2012; 109:318–324. © 2011 Wiley Periodicals, Inc.

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