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Metabolic characterization and genes for the conversion of biphenyl in Dyella ginsengisoli LA-4

Authors

  • Ang Li,

    1. State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, People's Republic of China; telephone: +86-411-84706251; fax: +86-411-84706252
    2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, People's Republic of China
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  • Yuan-Yuan Qu,

    Corresponding author
    1. State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, People's Republic of China; telephone: +86-411-84706251; fax: +86-411-84706252
    • State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, People's Republic of China; telephone: +86-411-84706251; fax: +86-411-84706252.
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  • Wen-Qing Pi,

    1. State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, People's Republic of China; telephone: +86-411-84706251; fax: +86-411-84706252
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  • Ji-Ti Zhou,

    1. State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, People's Republic of China; telephone: +86-411-84706251; fax: +86-411-84706252
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  • Zhong-Hui Gai,

    1. State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai 200240, People's Republic of China; telephone: +86-21-34206647; fax: +86-21-34206723
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  • Ping Xu

    Corresponding author
    1. State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai 200240, People's Republic of China; telephone: +86-21-34206647; fax: +86-21-34206723
    • State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University Shanghai 200240, People's Republic of China; telephone: +86-21-34206647; fax: +86-21-34206723.
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Abstract

A complete bph gene cluster (bphLA-4) containing 12,186 bp was amplified from Dyella ginsengisoli LA-4. The bphLA-4 was composed of bphABCXD, and an additional gene encoding a meta-fission product hydrolase was located in the bphX region. BphLA-4 was independently transcribed by the two operons, bphA1A2orf1A3A4BCX0 and bphX1orf2X2X3D, and significantly differed from bphKF707. Both benzoate and catechol induced the expression of both operons. 2-Hydroxypenta-2,4-dienoate was identified as the intermediate of the biphenyl degradation by strain LA-4. This finding suggested that there existed a novel lower pathway of biphenyl degradation in strain LA-4. Biotechnol. Bioeng. 2012; 109:609–613. © 2011 Wiley Periodicals, Inc.

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