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Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: SEM imaging (part 2)

Authors

  • Meijuan Zeng,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, Purdue University, 500 Central Drive, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022, fax: 765-494-7023
    2. Department of Agricultural and Biological Engineering, Purdue University, 225 South University Street, West Lafayette, Indiana 47907-2093
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  • Eduardo Ximenes,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, Purdue University, 500 Central Drive, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022, fax: 765-494-7023
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  • Michael R. Ladisch,

    Corresponding author
    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, Purdue University, 500 Central Drive, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022, fax: 765-494-7023
    2. Department of Agricultural and Biological Engineering, Purdue University, 225 South University Street, West Lafayette, Indiana 47907-2093
    3. Weldon School of Biomedical Engineering, Purdue University, 206 Martin Jischke Drive, West Lafayette, Indiana 47907-2032
    • Laboratory of Renewable Resources Engineering, Potter Engineering Center, Purdue University, 500 Central Drive, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022, fax: 765-494-7023.
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  • Nathan S. Mosier,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, Purdue University, 500 Central Drive, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022, fax: 765-494-7023
    2. Department of Agricultural and Biological Engineering, Purdue University, 225 South University Street, West Lafayette, Indiana 47907-2093
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  • Wilfred Vermerris,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, Purdue University, 500 Central Drive, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022, fax: 765-494-7023
    2. University of Florida Genetics Institute and Agronomy Department, Cancer/Genetics Research Complex, 2033 Mowry Road, Gainesville, Florida 32610
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  • Chia-Ping Huang,

    1. Life Science Microscopy Facility, Purdue University, S-052 Whistler Building, 170 S. University Street, West Lafayette, Indiana 47907-2072
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  • Debra M. Sherman

    1. Life Science Microscopy Facility, Purdue University, S-052 Whistler Building, 170 S. University Street, West Lafayette, Indiana 47907-2072
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  • Disclosure: M. R. Ladisch is CTO, Mascoma Corporation.

Abstract

In the first part of our work, we combined compositional analysis, pretreatment and enzyme hydrolysis for fractionated pith, rind, and leaf tissues from a hybrid stay-green corn, in order to identify the role of structural characteristics on enzyme hydrolysis of cell walls. Hydrolysis experiments coupled with chemical analysis of the different fractions of corn stover showed significant differences in cell wall structure before and after liquid hot water pretreatment. The extent of enzyme hydrolysis followed the sequence rind < leaves < pith with 90% conversion of cellulose to glucose in 24 h in the best cases. Since similar lignin contents remained after liquid hot water pretreatment of leaves, rind, and pith, our results indicated that the amount of lignin alone is not sufficient to explain the different enzymatic hydrolysis characteristics of the fractions. While the role of structural characteristics on enzyme hydrolysis of cell walls is measured as described in part I, the SEM images presented in this part II of our work show that sugar yields from enzymatic hydrolysis of corn fractions correlate with changes in plant cell wall structure both before and after liquid hot water pretreatment. Biotechnol. Bioeng. 2012; 109:398–404. © 2011 Wiley Periodicals, Inc.

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