Tissue-specific biomass recalcitrance in corn stover pretreated with liquid hot-water: Enzymatic hydrolysis (part 1)

Authors

  • Meijuan Zeng,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, 500 Central Drive, Purdue University, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022; fax: 765-494-7023
    2. Department of Agricultural and Biological Engineering, Purdue University, 225 South University Street, West Lafayette, Indiana 47907-2093
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  • Eduardo Ximenes,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, 500 Central Drive, Purdue University, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022; fax: 765-494-7023
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  • Michael R. Ladisch,

    Corresponding author
    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, 500 Central Drive, Purdue University, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022; fax: 765-494-7023
    2. Department of Agricultural and Biological Engineering, Purdue University, 225 South University Street, West Lafayette, Indiana 47907-2093
    3. Weldon School of Biomedical Engineering, Purdue University, 206 Martin Jischke Drive, West Lafayette, Indiana 47907-2032
    • Laboratory of Renewable Resources Engineering, Potter Engineering Center, 500 Central Drive, Purdue University, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022; fax: 765-494-7023.
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  • Nathan S. Mosier,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, 500 Central Drive, Purdue University, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022; fax: 765-494-7023
    2. Department of Agricultural and Biological Engineering, Purdue University, 225 South University Street, West Lafayette, Indiana 47907-2093
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  • Wilfred Vermerris,

    1. Laboratory of Renewable Resources Engineering, Potter Engineering Center, 500 Central Drive, Purdue University, West Lafayette, Indiana 47907-2022; telephone: 765-494-7022; fax: 765-494-7023
    2. University of Florida Genetics Institute and Agronomy Department, Cancer/Genetics Research Complex, 2033 Mowry Road, Gainesville, Florida 32610
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  • Chia-Ping Huang,

    1. Life Science Microscopy Facility, Purdue University, S-052 Whistler Building, 170 S. University Street, West Lafayette, Indiana 47907-2072
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  • Debra M. Sherman

    1. Life Science Microscopy Facility, Purdue University, S-052 Whistler Building, 170 S. University Street, West Lafayette, Indiana 47907-2072
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  • Disclosure: M. R. Ladisch is CTO, Mascoma Corporation.

Abstract

Lignin content, composition, distribution as well as cell wall thickness, structures, and type of tissue have a measurable effect on enzymatic hydrolysis of cellulose in lignocellulosic feedstocks. The first part of our work combined compositional analysis, pretreatment and enzyme hydrolysis for fractionated pith, rind, and leaf tissues from a hybrid stay-green corn, in order to identify the role of structural characteristics on enzyme hydrolysis of cell walls. The extent of enzyme hydrolysis follows the sequence rind < leaves < pith with 90% conversion of cellulose to glucose in 24 h in the best cases. Physical fractionation of corn stalks or other C4 grasses into soft and hard tissue types could reduce cost of cellulose conversion by enabling reduced enzyme loadings to hydrolyze soft tissue, and directing the hard tissue to other uses such as thermal processing, combustion, or recycle to the land from which the corn was harvested. Biotechnol. Bioeng. 2012; 109:390–397. © 2011 Wiley Periodicals, Inc.

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