Article

A modified IMAC method for the capture of target protein from mammalian cell culture harvest containing metal chelating species

  1. Aming Zhang1,†,
  2. Cheng Zhang2,
  3. Veena Warikoo2,
  4. Jonathan Forstrom3,
  5. Frank Riske2,*

Article first published online: 28 OCT 2011

DOI: 10.1002/bit.24353

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 109, Issue 3, pages 747–753, March 2012

Additional Information

How to Cite

Zhang, A., Zhang, C., Warikoo, V., Forstrom, J. and Riske, F. (2012), A modified IMAC method for the capture of target protein from mammalian cell culture harvest containing metal chelating species. Biotechnol. Bioeng., 109: 747–753. doi: 10.1002/bit.24353

Author Information

  1. 1

    Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia

  2. 2

    Purification Development, Genzyme Corporation, 45 New York Ave, Framingham, Massachusetts 01710; telephone: +508-271-3528; fax: +508-271-3454

  3. 3

    BioProcess Engineering, Genzyme Corporation, 200 Crossing Blvd, Framingham, Massachusetts

  1. †

    Process and Product Development, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA, 91320.

*Purification Development, Genzyme Corporation, 45 New York Ave, Framingham, Massachusetts 01710; telephone: +508-271-3528; fax: +508-271-3454.

Publication History

  1. Issue published online: 17 JAN 2012
  2. Article first published online: 28 OCT 2011
  3. Accepted manuscript online: 19 OCT 2011 10:27AM EST
  4. Manuscript Accepted: 14 OCT 2011
  5. Manuscript Revised: 7 OCT 2011
  6. Manuscript Received: 1 JUL 2011

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