Article

Inhibition of glutamine-dependent autophagy increases t-PA production in CHO Cell fed-batch processes

  1. Mario A. Jardon1,2,
  2. Beheroze Sattha3,
  3. Katrin Braasch4,
  4. Amy O. Leung5,
  5. Hélène C.F. Côté3,
  6. Michael Butler4,
  7. Sharon M. Gorski5,6,
  8. James M. Piret1,2,*

Article first published online: 21 DEC 2011

DOI: 10.1002/bit.24393

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 109, Issue 5, pages 1228–1238, May 2012

Additional Information

How to Cite

Jardon, M. A., Sattha, B., Braasch, K., Leung, A. O., Côté, H. C.F., Butler, M., Gorski, S. M. and Piret, J. M. (2012), Inhibition of glutamine-dependent autophagy increases t-PA production in CHO Cell fed-batch processes. Biotechnol. Bioeng., 109: 1228–1238. doi: 10.1002/bit.24393

Author Information

  1. 1

    Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC, Canada, V6T 1Z4. Tel.: 604-822-5835, Fax: 604-822-2114

  2. 2

    Department of Chemical and Biological Engineering, UBC, 2360 East Mall, Vancouver, BC, Canada, V6T 1Z3

  3. 3

    Department of Pathology and Laboratory Medicine, UBC, G227-2211 Wesbrook Mall, Vancouver, BC, Canada, V6T 2B5

  4. 4

    Department of Microbiology, University of Manitoba, 79 Freedman Crescent, Fort Garry Campus, Winnipeg, MB, Canada, R3T 2N2

  5. 5

    Michael Smith Genome Sciences Centre, BC Cancer Research Centre, 675 West 10 Ave, Vancouver, BC, V5Z 1L3

  6. 6

    Department of Molecular Biology and Biochemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada, V5A 1S6

*Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC, Canada, V6T 1Z4. Tel.: 604-822-5835, Fax: 604-822-2114.

Publication History

  1. Issue published online: 15 MAR 2012
  2. Article first published online: 21 DEC 2011
  3. Accepted manuscript online: 28 NOV 2011 08:48AM EST
  4. Manuscript Accepted: 16 NOV 2011
  5. Manuscript Revised: 6 OCT 2011
  6. Manuscript Received: 12 JUL 2011

Funded by

  • Natural Sciences and Engineering Research Council (NSERC)

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    Payel Datta, Robert J. Linhardt, Susan T. Sharfstein, An 'omics approach towards CHO cell engineering, Biotechnology and Bioengineering, 2013, 110, 5
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