Article

384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids

  1. Amy Y. Hsiao1,
  2. Yi-Chung Tung1,2,
  3. Xianggui Qu3,
  4. Lalit R. Patel1,4,
  5. Kenneth J. Pienta4,5,
  6. Shuichi Takayama1,6,7,*

Article first published online: 20 DEC 2011

DOI: 10.1002/bit.24399

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 109, Issue 5, pages 1293–1304, May 2012

Additional Information

How to Cite

Hsiao, A. Y., Tung, Y.-C., Qu, X., Patel, L. R., Pienta, K. J. and Takayama, S. (2012), 384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids. Biotechnol. Bioeng., 109: 1293–1304. doi: 10.1002/bit.24399

Author Information

  1. 1

    Department of Biomedical Engineering, University of Michigan, 2215 Carl A Gerstacker Bldg, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109; telephone: +1-734-615-5539; fax: +1-734-936-1905

  2. 2

    Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan

  3. 3

    Department of Mathematics and Statistics, Oakland University, Rochester, Michigan

  4. 4

    Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan

  5. 5

    Department of Urology, University of Michigan Medical School, Ann Arbor, Michigan

  6. 6

    Macro Molecular Science and Engineering, University of Michigan, Ann Arbor, Michigan

  7. 7

    School of Nano-Biotechnology and Chemical Engineering WCU Project, UNIST, Ulsan, Republic of Korea

*Department of Biomedical Engineering, University of Michigan, 2215 Carl A Gerstacker Bldg, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109; telephone: +1-734-615-5539; fax: +1-734-936-1905.

Publication History

  1. Issue published online: 15 MAR 2012
  2. Article first published online: 20 DEC 2011
  3. Accepted manuscript online: 7 DEC 2011 09:56AM EST
  4. Manuscript Accepted: 23 NOV 2011
  5. Manuscript Revised: 21 NOV 2011
  6. Manuscript Received: 22 SEP 2011

Funded by

  • Coulter Foundation
  • College of Engineering Translational Research Fund
  • University of Michigan
  • NIH. Grant Number: 1 PO1 CA093900

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Keywords:

  • spheroid;
  • Z-factor;
  • high-throughput;
  • 3D;
  • hanging drop

Abstract

We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practical 3D cell-based high-throughput screening and enable broader use of the plate, we characterize the robustness of the 384 hanging drop array plate in terms of assay performance and demonstrate the versatility of the plate. We find that the 384 hanging drop array plate performance is robust in fluorescence- and colorimetric-based assays through Z-factor calculations. Finally, we demonstrate different plate capabilities and applications, including: spheroid transfer and retrieval for Janus spheroid formation, sequential addition of cells for concentric layer patterning of different cell types, and culture of a wide variety of cell types. Biotechnol. Bioeng. 2012; 109:1293–1304. © 2011 Wiley Periodicals, Inc.

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