384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids

Authors

  • Amy Y. Hsiao,

    1. Department of Biomedical Engineering, University of Michigan, 2215 Carl A Gerstacker Bldg, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109; telephone: +1-734-615-5539; fax: +1-734-936-1905
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  • Yi-Chung Tung,

    1. Department of Biomedical Engineering, University of Michigan, 2215 Carl A Gerstacker Bldg, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109; telephone: +1-734-615-5539; fax: +1-734-936-1905
    2. Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan
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  • Xianggui Qu,

    1. Department of Mathematics and Statistics, Oakland University, Rochester, Michigan
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  • Lalit R. Patel,

    1. Department of Biomedical Engineering, University of Michigan, 2215 Carl A Gerstacker Bldg, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109; telephone: +1-734-615-5539; fax: +1-734-936-1905
    2. Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan
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  • Kenneth J. Pienta,

    1. Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan
    2. Department of Urology, University of Michigan Medical School, Ann Arbor, Michigan
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  • Shuichi Takayama

    Corresponding author
    1. Department of Biomedical Engineering, University of Michigan, 2215 Carl A Gerstacker Bldg, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109; telephone: +1-734-615-5539; fax: +1-734-936-1905
    2. Macro Molecular Science and Engineering, University of Michigan, Ann Arbor, Michigan
    3. School of Nano-Biotechnology and Chemical Engineering WCU Project, UNIST, Ulsan, Republic of Korea
    • Department of Biomedical Engineering, University of Michigan, 2215 Carl A Gerstacker Bldg, 2200 Bonisteel Blvd, Ann Arbor, Michigan 48109; telephone: +1-734-615-5539; fax: +1-734-936-1905.
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Abstract

We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practical 3D cell-based high-throughput screening and enable broader use of the plate, we characterize the robustness of the 384 hanging drop array plate in terms of assay performance and demonstrate the versatility of the plate. We find that the 384 hanging drop array plate performance is robust in fluorescence- and colorimetric-based assays through Z-factor calculations. Finally, we demonstrate different plate capabilities and applications, including: spheroid transfer and retrieval for Janus spheroid formation, sequential addition of cells for concentric layer patterning of different cell types, and culture of a wide variety of cell types. Biotechnol. Bioeng. 2012; 109:1293–1304. © 2011 Wiley Periodicals, Inc.

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