Marketa Hnilova and Banu Taktak Karaca contributed equally to this work.
Fabrication of hierarchical hybrid structures using bio-enabled layer-by-layer self-assembly†
Article first published online: 26 DEC 2011
Copyright © 2011 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 109, Issue 5, pages 1120–1130, May 2012
How to Cite
Hnilova, M., Karaca, B. T., Park, J., Jia, C., Wilson, B. R., Sarikaya, M. and Tamerler, C. (2012), Fabrication of hierarchical hybrid structures using bio-enabled layer-by-layer self-assembly. Biotechnol. Bioeng., 109: 1120–1130. doi: 10.1002/bit.24405
- Issue published online: 15 MAR 2012
- Article first published online: 26 DEC 2011
- Accepted manuscript online: 14 DEC 2011 09:07AM EST
- Manuscript Accepted: 28 NOV 2011
- Manuscript Revised: 15 NOV 2011
- Manuscript Received: 11 JUL 2011
- NSF-MRSEC (GEMSEC) Program. Grant Number: DMR#0520567
- Turkish State Planning Organization
- NSF-BioMat Program. Grant Number: DMR#0706655
- bio-enabled microarrays;
- gold nanoparticle;
- hetero-functional protein;
- layer-by-layer self-assembly;
- solid recognition
Development of versatile and flexible assembly systems for fabrication of functional hybrid nanomaterials with well-defined hierarchical and spatial organization is of a significant importance in practical nanobiotechnology applications. Here we demonstrate a bio-enabled self-assembly technique for fabrication of multi-layered protein and nanometallic assemblies utilizing a modular gold-binding (AuBP1) fusion tag. To accomplish the bottom-up assembly we first genetically fused the AuBP1 peptide sequence to the C′-terminus of maltose-binding protein (MBP) using two different linkers to produce MBP-AuBP1 hetero-functional constructs. Using various spectroscopic techniques, surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR), we verified the exceptional binding and self-assembly characteristics of AuBP1 peptide. The AuBP1 peptide tag can direct the organization of recombinant MBP protein on various gold surfaces through an efficient control of the organic–inorganic interface at the molecular level. Furthermore using a combination of soft-lithography, self-assembly techniques and advanced AuBP1 peptide tag technology, we produced spatially and hierarchically controlled protein multi-layered assemblies on gold nanoparticle arrays with high molecular packing density and pattering efficiency in simple, reproducible steps. This model system offers layer-by-layer assembly capability based on specific AuBP1 peptide tag and constitutes novel biological routes for biofabrication of various protein arrays, plasmon-active nanometallic assemblies and devices with controlled organization, packing density and architecture. Biotechnol. Bioeng. 2012; 109:1120–1130. © 2011 Wiley Periodicals, Inc.