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Consolidated bioprocessing (CBP) of AFEX™-pretreated corn stover for ethanol production using Clostridium phytofermentans at a high solids loading

Authors

  • Mingjie Jin,

    Corresponding author
    1. Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, University Corporate Research Complex, 3900 Collins Road, Lansing, Michigan 48910; telephone: 517 432 0157; fax: +1 517 336 4615
    2. Great Lakes Bioenergy Research Center (GLBRC), Michigan State University, East Lansing, Michigan
    • Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, University Corporate Research Complex, 3900 Collins Road, Lansing, Michigan 48910; telephone: 517 432 0157; fax: +1 517 336 4615.
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  • Christa Gunawan,

    1. Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, University Corporate Research Complex, 3900 Collins Road, Lansing, Michigan 48910; telephone: 517 432 0157; fax: +1 517 336 4615
    2. Great Lakes Bioenergy Research Center (GLBRC), Michigan State University, East Lansing, Michigan
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  • Venkatesh Balan,

    1. Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, University Corporate Research Complex, 3900 Collins Road, Lansing, Michigan 48910; telephone: 517 432 0157; fax: +1 517 336 4615
    2. Great Lakes Bioenergy Research Center (GLBRC), Michigan State University, East Lansing, Michigan
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  • Bruce E. Dale

    1. Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, University Corporate Research Complex, 3900 Collins Road, Lansing, Michigan 48910; telephone: 517 432 0157; fax: +1 517 336 4615
    2. Great Lakes Bioenergy Research Center (GLBRC), Michigan State University, East Lansing, Michigan
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  • AFEX is a Trademark of MBI, International.

Abstract

Consolidated bioprocessing (CBP) using Clostridium phytofermentans (ATCC 700394) on ammonia fiber expansion (AFEX™)-treated corn stover (AFEX™-CS) at a low solids loading showed promising results [Jin et al. (2011) Biotechnol Bioeng 108(6): 1290–1297]. However, industrial relevant process requires high solids loading. Therefore, we studied high solids loading CBP performance on AFEX™-CS. The factors potentially affecting the performance including solids loading, CBP products acetate and ethanol, and degradation products resulting from pretreatment were investigated. At 4% (w/w) glucan loading, C. phytofermentans performed well on AFEX™-CS with no nutrients supplementation and reached similar sugar conversions as a fermentation with nutrients supplementation. A glucan conversion of 48.9% and a xylan conversion of 77.9% were achieved after 264 h with 7.0 g/L ethanol and 8.8 g/L acetate produced. Relatively high concentrations of acetate produced at high solids loading was found to be the major factor limiting the CBP performance. Degradation products in AFEX™-CS helped enhance ethanol production. Biotechnol. Bioeng. 2012; 109:1929–1936. © 2012 Wiley Periodicals, Inc.

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