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Differential effects of nitrogen and sulfur deprivation on growth and biodiesel feedstock production of Chlamydomonas reinhardtii

Authors

  • Turgay Cakmak,

    1. Laboratory of Sustainable Technologies, UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey; telephone: +90-312-290-3573; fax: +90-312-266-4365
    2. Faculty of Science, Department of Molecular Biology and Genetics, Istanbul Medeniyet University, 34730, Istanbul, Turkey
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  • Pinar Angun,

    1. Laboratory of Sustainable Technologies, UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey; telephone: +90-312-290-3573; fax: +90-312-266-4365
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  • Yunus Emre Demiray,

    1. Laboratory of Sustainable Technologies, UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey; telephone: +90-312-290-3573; fax: +90-312-266-4365
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  • Alper Devrim Ozkan,

    1. Laboratory of Sustainable Technologies, UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey; telephone: +90-312-290-3573; fax: +90-312-266-4365
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  • Zeynep Elibol,

    1. Laboratory of Sustainable Technologies, UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey; telephone: +90-312-290-3573; fax: +90-312-266-4365
    2. Faculty of Arts and Sciences, Department of Biology, Kırıkkale University, 71450, Kırıkkale, Turkey
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  • Turgay Tekinay

    Corresponding author
    1. Laboratory of Sustainable Technologies, UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey; telephone: +90-312-290-3573; fax: +90-312-266-4365
    • Laboratory of Sustainable Technologies, UNAM Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey; telephone: +90-312-290-3573; fax: +90-312-266-4365.
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Abstract

Biodiesel production from microalgae is a promising approach for energy production; however, high cost of its process limits the use of microalgal biodiesel. Increasing the levels of triacylglycerol (TAG) levels, which is used as a biodiesel feedstock, in microalgae has been achieved mainly by nitrogen starvation. In this study, we compared effects of sulfur (S) and nitrogen (N) starvation on TAG accumulation and related parameters in wild-type Chlamydomonas reinhardtii CC-124 mt(−) and CC-125 mt(+) strains. Cell division was interrupted, protein and chlorophyll levels rapidly declined while cell volume, total neutral lipid, carotenoid, and carbohydrate content increased in response to nutrient starvation. Cytosolic lipid droplets in microalgae under nutrient starvation were monitored by three-dimensional confocal laser imaging of live cells. Infrared spectroscopy results showed that relative TAG, oligosaccharide and polysaccharide levels increased rapidly in response to nutrient starvation, especially S starvation. Both strains exhibited similar levels of regulation responses under mineral deficiency, however, the degree of their responses were significantly different, which emphasizes the importance of mating type on the physiological response of algae. Neutral lipid, TAG, and carbohydrate levels reached their peak values following 4 days of N or S starvation. Therefore, 4 days of N or S starvation provides an excellent way of increasing TAG content. Although increase in these parameters was followed by a subsequent decline in N-starved strains after 4 days, this decline was not observed in S-starved ones, which shows that S starvation is a better way of increasing TAG production of C. reinhardtii than N starvation. Biotechnol. Bioeng. 2012; 109:1947–1957. © 2012 Wiley Periodicals, Inc.

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