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Keywords:

  • high throughput;
  • electroporation;
  • protocol design

Abstract

The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system. Biotechnol. Bioeng. 2012; 109:2168–2171. © 2012 Wiley Periodicals, Inc.