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Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Authors

  • Francois Fernand,

    1. Department of Mechanical Engineering, Etcheverry Hall, 6124, University of California at Berkeley, 2521 Hearst Ave, Berkeley, California 94720; telephone: 510-221-6557; fax: 510-642-6163
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  • Liel Rubinsky,

    1. Department of Mechanical Engineering, Etcheverry Hall, 6124, University of California at Berkeley, 2521 Hearst Ave, Berkeley, California 94720; telephone: 510-221-6557; fax: 510-642-6163
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  • Alex Golberg,

    Corresponding author
    1. Department of Mechanical Engineering, Etcheverry Hall, 6124, University of California at Berkeley, 2521 Hearst Ave, Berkeley, California 94720; telephone: 510-221-6557; fax: 510-642-6163
    • Department of Mechanical Engineering, Etcheverry Hall, 6124, University of California at Berkeley, 2521 Hearst Ave, Berkeley, California 94720; telephone: 510-221-6557; fax: 510-642-6163.
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  • Boris Rubinsky

    1. Department of Mechanical Engineering, Etcheverry Hall, 6124, University of California at Berkeley, 2521 Hearst Ave, Berkeley, California 94720; telephone: 510-221-6557; fax: 510-642-6163
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Abstract

The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system. Biotechnol. Bioeng. 2012; 109:2168–2171. © 2012 Wiley Periodicals, Inc.

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