Improving piezoelectric cell printing accuracy and reliability through neutral buoyancy of suspensions

Authors

  • Daljeet Chahal,

    Corresponding author
    1. Electrical and Computer Engineering, University of British Columbia, 4060 - 2332 Main Mall, Vancouver, BC, Canada V6T 1Z4; telephone: 604-754-5658; fax: 604-822-5949
    • Electrical and Computer Engineering, University of British Columbia, 4060 - 2332 Main Mall, Vancouver, BC, Canada V6T 1Z4; telephone: 604-754-5658; fax: 604-822-5949.
    Search for more papers by this author
  • Ali Ahmadi,

    1. Electrical and Computer Engineering, University of British Columbia, 4060 - 2332 Main Mall, Vancouver, BC, Canada V6T 1Z4; telephone: 604-754-5658; fax: 604-822-5949
    Search for more papers by this author
  • Karen C. Cheung

    1. Electrical and Computer Engineering, University of British Columbia, 4060 - 2332 Main Mall, Vancouver, BC, Canada V6T 1Z4; telephone: 604-754-5658; fax: 604-822-5949
    Search for more papers by this author

Abstract

The sedimentation and aggregation of cells within inkjet printing systems has been hypothesized to negatively impact printer performance. The purpose of this study was to investigate this influence through the use of neutral buoyancy. Ficoll PM400 was used to create neutrally buoyant MCF-7 breast cancer cell suspensions, which were ejected using a piezoelectric drop-on-demand inkjet printing system. It was found that using a neutrally buoyant suspension greatly increased the reproducibility of consistent cell counts, and eliminated nozzle clogging. Moreover, the use of Ficoll PM400 was shown to not affect cellular viability. This is the first demonstration of such scale and accuracy achieved using a piezoelectric inkjet printing system for cellular dispensing. Biotechnol. Bioeng. 2012; 109: 2932–2940. © 2012 Wiley Periodicals, Inc.

Ancillary