Conflict of interest: Nothing to declare.
Design of Ca2+-independent Staphylococcus aureus sortase A mutants†
Version of Record online: 4 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 109, Issue 12, pages 2955–2961, December 2012
How to Cite
Hirakawa, H., Ishikawa, S. and Nagamune, T. (2012), Design of Ca2+-independent Staphylococcus aureus sortase A mutants. Biotechnol. Bioeng., 109: 2955–2961. doi: 10.1002/bit.24585
- Issue online: 25 OCT 2012
- Version of Record online: 4 JUL 2012
- Accepted manuscript online: 21 JUN 2012 08:50AM EST
- Manuscript Accepted: 11 JUN 2012
- Manuscript Revised: 3 JUN 2012
- Manuscript Received: 14 APR 2012
Additional Supporting Information may be found in the online version of this article.
|bit_24585_sm_SupplFig1.doc||202K||Supplementary Figure 1. Kinetic measurements of the reaction between Abz-LPETGK(Dnp)- CONH2 and 1 mM triglycine to yield Abz-LPETGGG catalyzed by (A) wild-type SaSrtA, (B) the E105K/E108A mutant, and (C) the E105K/E108Q mutant. The reactions were conducted in the presence of 10 mM Ca2+ (closed circle) or without Ca2+ (open circle) at 25°C.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.