On-demand killing of adherent cells on photo-acid-generating culture substrates

Authors

  • Kimio Sumaru,

    Corresponding author
    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
    • Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
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  • Kyoko Kikuchi,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
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  • Toshiyuki Takagi,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
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  • Manae Yamaguchi,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
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  • Taku Satoh,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
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  • Kana Morishita,

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
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  • Toshiyuki Kanamori

    1. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan; telephone: +81-29-861-6373; fax: +81-29-861-6278
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Abstract

As a powerful tool of cell screening and cell purification, we developed a novel method to kill adherent cells as cultured on a substrate by micro-projection of incoherent visible light. To kill the cells by the mild light irradiated by electrically controllable micro-projection systems currently available, we introduced the assist of the photo-responsive culture substrates functionalized with a photo-acid-generating polymer. In clear contrast to the existing laser-based methods requiring point scanning, areal micro-prjection of blue light with the wavelength 436 nm killed many CHO-K1 cells at a time in the irradiated area on the substrate. The effect of the photo-generated acid was so confined that selective killing of targeted cells was achieved without critical damage to the neighboring cells. Further, we demonstrated the photo-selective killing of the adherent cells after preliminarily patterning through the photo-induced removal of cell adhesion-inhibiting polymer. Biotechnol. Bioeng. 2013; 110: 348–352. © 2012 Wiley Periodicals, Inc.

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