The authors have no conflict of interest to declare.
Simultaneous clostridial fermentation, lipase-catalyzed esterification, and ester extraction to enrich diesel with butyl butyrate†
Article first published online: 22 AUG 2012
Copyright © 2012 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 110, Issue 1, pages 137–142, January 2013
How to Cite
van den Berg, C., Heeres, A. S., van der Wielen, L. A. M. and Straathof, A. J. J. (2013), Simultaneous clostridial fermentation, lipase-catalyzed esterification, and ester extraction to enrich diesel with butyl butyrate. Biotechnol. Bioeng., 110: 137–142. doi: 10.1002/bit.24618
- Issue published online: 20 NOV 2012
- Article first published online: 22 AUG 2012
- Accepted manuscript online: 25 JUL 2012 10:16AM EST
- Manuscript Accepted: 16 JUL 2012
- Manuscript Revised: 9 JUL 2012
- Manuscript Received: 27 APR 2012
The recovery of 1-butanol from fermentation broth is energy-intensive since typical concentrations in fermentation broth are below 20 g L−1. To prevent butanol inhibition and high downstream processing costs, we aimed at producing butyl esters instead of 1-butanol. It is shown that it is possible to perform simultaneously clostridial fermentation, esterification of the formed butanol to butyl butyrate, and extraction of this ester by hexadecane. The very high partition coefficient of butyl butyrate pulls the esterification towards the product side even at fermentation pH and relatively low butanol concentrations. The hexadecane extractant is a model diesel compound and is nontoxic to the cells. If butyl butyrate enriched diesel can directly be used as car fuel, no product recovery is required. A proof-of-principle experiment for the one-pot bio-ester production from glucose led to 5 g L−1 butyl butyrate in the hexadecane phase. The principle may be extended to a wide range of esters, especially to longer chain ones. Biotechnol. Bioeng. 2013; 110: 137–142. © 2012 Wiley Periodicals, Inc.