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Simultaneous clostridial fermentation, lipase-catalyzed esterification, and ester extraction to enrich diesel with butyl butyrate

Authors

  • Corjan van den Berg,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; telephone: 31-15-2782330; fax: 31-15-2782355
    Current affiliation:
    1. Present address: TNO Earth Environment & Life Sciences, Utrechtseweg 48, 3704 HE Zeist, The Netherlands.
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  • Arjan S. Heeres,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; telephone: 31-15-2782330; fax: 31-15-2782355
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  • Luuk A. M. van der Wielen,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; telephone: 31-15-2782330; fax: 31-15-2782355
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  • Adrie J. J. Straathof

    Corresponding author
    1. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; telephone: 31-15-2782330; fax: 31-15-2782355
    • Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands; telephone: 31-15-2782330; fax: 31-15-2782355
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  • The authors have no conflict of interest to declare.

Abstract

The recovery of 1-butanol from fermentation broth is energy-intensive since typical concentrations in fermentation broth are below 20 g L−1. To prevent butanol inhibition and high downstream processing costs, we aimed at producing butyl esters instead of 1-butanol. It is shown that it is possible to perform simultaneously clostridial fermentation, esterification of the formed butanol to butyl butyrate, and extraction of this ester by hexadecane. The very high partition coefficient of butyl butyrate pulls the esterification towards the product side even at fermentation pH and relatively low butanol concentrations. The hexadecane extractant is a model diesel compound and is nontoxic to the cells. If butyl butyrate enriched diesel can directly be used as car fuel, no product recovery is required. A proof-of-principle experiment for the one-pot bio-ester production from glucose led to 5 g L−1 butyl butyrate in the hexadecane phase. The principle may be extended to a wide range of esters, especially to longer chain ones. Biotechnol. Bioeng. 2013; 110: 137–142. © 2012 Wiley Periodicals, Inc.

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