Author's contribution: ARB and KRP conceived the study. ARB designed and carried out the experiments. ARB and KRP analyzed the results and wrote the manuscript.
Communication to the Editor
Overexpression of O-methyltransferase leads to improved vanillin production in baker's yeast only when complemented with model-guided network engineering†
Article first published online: 23 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Biotechnology and Bioengineering
Volume 110, Issue 2, pages 656–659, February 2013
How to Cite
Brochado, A. R. and Patil, K. R. (2013), Overexpression of O-methyltransferase leads to improved vanillin production in baker's yeast only when complemented with model-guided network engineering. Biotechnol. Bioeng., 110: 656–659. doi: 10.1002/bit.24731
- Issue published online: 20 DEC 2012
- Article first published online: 23 OCT 2012
- Accepted manuscript online: 24 SEP 2012 07:27AM EST
- Manuscript Accepted: 10 SEP 2012
- Manuscript Revised: 12 AUG 2012
- Manuscript Received: 11 MAY 2012
- Fundação para a Ciência e Tecnologia. Grant Number: SFRH/BD/41230/2007
- Saccharomyces cerevisiae;
- pathway engineering
Overproduction of a desired metabolite is often achieved via manipulation of the pathway directly leading to the product or through engineering of distant nodes within the metabolic network. Empirical examples illustrating the combined effect of these local and global strategies have been so far limited in eukaryotic systems. In this study, we compared the effects of overexpressing a key gene in de novo vanillin biosynthesis (coding for O-methyltransferase, hsOMT) in two yeast strains, with and without model-guided global network modifications. Overexpression of hsOMT resulted in increased vanillin production only in the strain with model-guided modifications, exemplifying advantage of using a global strategy prior to local pathway manipulation. Biotechnol. Bioeng. 2013; 110: 656–659. © 2012 Wiley Periodicals, Inc.