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Reconstitution of cellulose and lignin after [C2mim][OAc] pretreatment and its relation to enzymatic hydrolysis

  1. Tong-Qi Yuan1,
  2. Wei Wang2,
  3. Li-Ming Zhang1,
  4. Feng Xu1,
  5. Run-Cang Sun1,3,*

Article first published online: 18 OCT 2012

DOI: 10.1002/bit.24743

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 110, Issue 3, pages 729–736, March 2013

Additional Information

How to Cite

Yuan, T.-Q., Wang, W., Zhang, L.-M., Xu, F. and Sun, R.-C. (2013), Reconstitution of cellulose and lignin after [C2mim][OAc] pretreatment and its relation to enzymatic hydrolysis. Biotechnol. Bioeng., 110: 729–736. doi: 10.1002/bit.24743

Author Information

  1. 1

    Institute of Biomass Chemistry and Technology, Beijing Forestry University, Beijing 100083, China; telephone: +86-10-62336903; fax: +86-10-62336903

  2. 2

    Institute of Microbiology, Beijing Forestry University, Beijing, China

  3. 3

    State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China

*Institute of Biomass Chemistry and Technology, Beijing Forestry University, Beijing 100083, China; telephone: +86-10-62336903; fax: +86-10-62336903.

Publication History

  1. Issue published online: 18 JAN 2013
  2. Article first published online: 18 OCT 2012
  3. Accepted manuscript online: 5 OCT 2012 07:28AM EST
  4. Manuscript Accepted: 25 SEP 2012
  5. Manuscript Revised: 30 JUL 2012
  6. Manuscript Received: 6 MAY 2012

Funded by

  • National Science Foundation of China. Grant Number: 31110103902
  • Major State Basic Research Projects of China. Grant Number: 973-2010CB732204
  • State Forestry Administration. Grant Number: 20100400706
  • China Ministry of Education. Grant Number: 111

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Keywords:

  • cellulose;
  • lignin;
  • enzymatic hydrolysis;
  • ionic liquid;
  • pretreatment

Abstract

Although the effects of cellulose crystallinity and lignin content as two major structural features on enzymatic hydrolysis have been extensively studied, debates regarding their effects still exist. In this study, reconstitution of cellulose and lignin after 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]) pretreatment was proposed as a new method to study their effects on enzymatic digestibility. Different mechanisms of lignin content for reduction of cellulose hydrolysis were found between the proposed method and the traditional method (mixing of cellulose and lignin). The results indicated that a slight change of the crystallinity of the reconstituted materials may play a minor role in the change of enzyme efficiency. In addition, the present study suggested that the lignin content does not significantly affect the digestibility of cellulose, whereas the conversion of cellulose fibers from the cellulose I to the cellulose II crystal phase plays an important role when an ionic liquid pretreatment of biomass was conducted. Biotechnol. Bioeng. 2013; 110: 729–736. © 2012 Wiley Periodicals, Inc.

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